Institute of Physical Chemistry , University of Freiburg , Freiburg , Baden-Württemberg 79104 , Germany.
J Phys Chem B. 2018 Dec 13;122(49):11373-11380. doi: 10.1021/acs.jpcb.8b07301. Epub 2018 Sep 13.
Hsp90 is an essential molecular chaperone, which has to be in a dimeric form for its correct function. While the affinity of the dimer has previously been measured, little is known about how it associates and dissociates and the factors that influence this. We perform an in-depth single molecule characterization of the C-terminal association and dissociation of Hsp90. We find more than one dissociation rate, indicating that the dimer has a stable and an unstable state. Furthermore, we find that the stability of the C-terminal association is dependent on the presence of ATP, despite the C-terminal dimerization interface being distal to the catalytic site.
Hsp90 是一种必需的分子伴侣，其正确功能需要以二聚体的形式存在。虽然之前已经测量了二聚体的亲和力，但对于其如何结合和解离以及影响因素知之甚少。我们对 Hsp90 的 C 端结合和解离进行了深入的单分子特性分析。我们发现了不止一个解离速率，这表明二聚体具有稳定和不稳定两种状态。此外，我们发现尽管 C 端二聚化界面远离催化位点，但 C 端结合的稳定性依赖于 ATP 的存在。
