Giaever G, Lynn R, Goto T, Wang J C
J Biol Chem. 1986 Sep 25;261(27):12448-54.
The nucleotide sequence of the gene TOP2 encoding DNA topoisomerase II of the yeast Saccharomyces cerevisiae has been determined. The entire coding sequence of the gene lies within an open reading frame, and there are 1429 amino acids in the single polypeptide protein if translation is assumed to start at the first ATG in this frame. The calculated subunit and molecular mass of the enzyme is 164,000 and 328,000 daltons, respectively. The transcriptional starts of the gene and a polyadenylation site of the message have also been mapped.
已经确定了酿酒酵母DNA拓扑异构酶II的编码基因TOP2的核苷酸序列。该基因的整个编码序列位于一个开放阅读框内，如果假定翻译从该阅读框中的第一个ATG开始，那么该单多肽蛋白中有1429个氨基酸。该酶计算得出的亚基分子量和分子量分别为164,000道尔顿和328,000道尔顿。还绘制了该基因的转录起始位点和该信使RNA的一个聚腺苷酸化位点。
