Civil Engineering, College of Engineering and Informatics, National University of Ireland, Galway, Ireland.
Zhejiang Provincial Key Laboratory of Water Science and Technology, Department of Environment, Yangtze Delta Region Institute of Tsinghua University, Zhejiang Province, China.
Sci Total Environ. 2019 Jan 10;647:1188-1198. doi: 10.1016/j.scitotenv.2018.08.098. Epub 2018 Aug 7.
This study investigated the performance and microbial community dynamics of a start-up method for the partial nitritation-anammox (PN-A) process: start-up from return sludge in an intermittently aerated sequencing batch reactor (IASBR). The robustness of this PN-A IASBR system in achieving long-term efficient nitrogen removal was also investigated. Stable partial nitritation with nitrite accumulation ratio of about 80% was firstly achieved in the IASBR. Then, PN-A process with total nitrogen removal of up to 81.5% was established due to the thriving of anammox bacteria Candidatus Kuenenia resulting from the reduction of the aeration rate. Molecular analysis showed that both bacterial and archaeal communities shifted greatly throughout the start-up stage and the PN-A stage. Besides bacterial genus Nitrosomonas, ammonium-oxidizing archaea (AOA) Candidatus Nitrososphaera with a high abundance of 3.44% also contributed to partial nitritation. Nitrospira was effectively restrained (abundance <1.6%) while methanogens co-existed with the aerobic and anaerobic nitrogen-conversion microorganisms. This study showed that IASBR configuration was efficient in starting up the PN-A process from return sludge, maintaining long-term efficient nitrogen removal and triggering the thrive of AOA.
本研究考察了一种部分亚硝化-厌氧氨氧化（PN-A）工艺启动方法的性能和微生物群落动态：在间歇曝气序批式反应器（IASBR）中从回流污泥启动。还研究了这种 PN-A IASBR 系统实现长期高效脱氮的稳健性。IASBR 中首先实现了稳定的部分亚硝化，亚硝酸盐积累比约为 80%。然后，由于曝气率降低，导致厌氧氨氧化菌 Candidatus Kuenenia 的大量繁殖，建立了总氮去除率高达 81.5%的 PN-A 工艺。分子分析表明，在启动阶段和 PN-A 阶段，细菌和古菌群落都发生了很大的变化。除了细菌属 Nitrosomonas 外，丰度高达 3.44%的氨氧化古菌（AOA）Candidatus Nitrososphaera 也有助于部分亚硝化。有效抑制了硝化菌（丰度<1.6%），而产甲烷菌与好氧和厌氧氮转化微生物共存。本研究表明，IASBR 构型可有效地从回流污泥中启动 PN-A 工艺，维持长期高效的脱氮，并促进 AOA 的大量繁殖。
