Felt V, Husek P
Endocrinol Exp. 1977;11(2):115-24.
The uptake of triiodothyronine (T3) and thyroxine (T4) by human leucocytes as well as by adipose and muscular tissues in vitro was markedly dependent on the extracellular protein concentration. The presence of extracellular protein was responsible for the difference in the uptake of T3 and T4 by leucocytes (the incorporation of T4 was significantly lower than that of T3), whereas its presence in the medium containing adipocytes did nto influence the incorporation values. In a protein-free medium, the muscular tissue uptake of T3 exceeded that of T4. In this tissue the deiodination of both hormones was likewise significantly different, that of T4 being higher. The conversion of T4 to T3 was also higher in the muscle cells, but represented only about 75% of the total deiodinated fraction. In the remaining tissues, no differences were observed in the uptake and deiodination of T3 and T4. The deiodination of T4 in human leucocytes was found to be considerably dependent of pH of the incubation medium (two deiodination optima, a higher one in acid region) whereas the deiodination of T3 was fairly indifferent to variations of pH (one flat deiodination optimum in the weakly alkaline region).
人白细胞以及体外脂肪组织和肌肉组织对三碘甲状腺原氨酸（T3）和甲状腺素（T4）的摄取明显取决于细胞外蛋白质浓度。细胞外蛋白质的存在导致白细胞对T3和T4摄取的差异（T4的掺入明显低于T3），而其在含有脂肪细胞的培养基中的存在并不影响掺入值。在无蛋白培养基中，肌肉组织对T3的摄取超过T4。在该组织中，两种激素的脱碘同样存在显著差异，T4的脱碘更高。肌肉细胞中T4向T3的转化也更高，但仅占总脱碘部分的约75%。在其余组织中，未观察到T3和T4摄取及脱碘的差异。发现人白细胞中T4的脱碘很大程度上取决于孵育培养基的pH值（有两个脱碘最佳值，在酸性区域较高），而T3的脱碘对pH值变化相当不敏感（在弱碱性区域有一个平缓的脱碘最佳值）。
