Aquatic Lab, Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Environ Toxicol Chem. 2018 Dec;37(12):3063-3076. doi: 10.1002/etc.4266. Epub 2018 Nov 19.
Although the effects of nano-sized titania (nTiO ) on hatching events (change in hatching time and total hatching) in zebrafish have been reported, additional consequences of nTiO exposure (i.e., the effects of nTiO -induced changes in hatching events and morphometric parameters on embryo-larvae development and survivability) have not been reported. To address this knowledge gap, embryos 4 h postfertilization were exposed to nTiO (0, 0.01, 10, and 1000 μg/mL) for 220 h. Hatching rate (58, 82, and 106 h postexposure [hpe]), survival rate (8 times from 34 to 202 hpe), and 21 morphometric characteristics (8 times from 34 to 202 hpe) were recorded. Total hatching (rate at 106 hpe) was significantly and positively correlated to survival rate, but there was no direct association between nTiO -induced change in hatching time (hatching rate at 58 and 82 hpe) and survival rate. At 58, 82, and 106 hpe, morphometric characteristics were significantly correlated to hatching rate, suggesting that the nTiO -induced change in hatching time can affect larval development. The morphometric characteristics that were associated with change in hatching time were also significantly correlated to survival rate, suggesting an indirect significant influence of the nTiO -induced change in hatching time on survivability. These results show a significant influence of nTiO -induced change in hatching events on zebrafish embryo-larvae development and survivability. They also show that morphometric maldevelopments can predict later-in-life consequences (survivability) of an embryonic exposure to nTiO . This suggests that zebrafish can be sensitive biological predictors of nTiO acute toxicity. Environ Toxicol Chem 2018;37:3063-3076. © 2018 SETAC.
尽管已有研究报道了纳米二氧化钛（nTiO ）对斑马鱼孵化事件（孵化时间和总孵化率的变化）的影响，但 nTiO 暴露的其他后果（即 nTiO 诱导的孵化事件变化和形态参数对胚胎-幼虫发育和存活率的影响）尚未报道。为了填补这一知识空白，将受精后 4 小时的胚胎暴露于 nTiO （0、0.01、10 和 1000μg/mL）中 220 小时。记录孵化率（暴露后 58、82 和 106 小时）、存活率（8 次从 34 小时到 202 小时）和 21 个形态特征（8 次从 34 小时到 202 小时）。总孵化率（106 小时时的孵化率）与存活率呈显著正相关，但孵化时间（58 和 82 小时时的孵化率）与存活率之间没有直接关联。在 58、82 和 106 小时时，形态特征与孵化率显著相关，表明 nTiO 诱导的孵化时间变化会影响幼虫发育。与孵化时间变化相关的形态特征也与存活率显著相关，表明 nTiO 诱导的孵化时间变化对存活率有间接显著影响。这些结果表明，nTiO 诱导的孵化事件变化对斑马鱼胚胎-幼虫发育和存活率有显著影响。它们还表明，形态畸形发育可以预测胚胎暴露于 nTiO 后的后期生存后果（存活率）。这表明，斑马鱼可能是 nTiO 急性毒性的敏感生物预测因子。Environ Toxicol Chem 2018;37:3063-3076. © 2018 SETAC.
