Wood R D, Stein J
Mol Gen Genet. 1986 Jul;204(1):82-4. doi: 10.1007/BF00330191.
E. coli recF mutants have a greatly reduced capacity for Weigle mutagenesis of ultraviolet light-irradiated lambda phage. A recF 332::Tn3 mutation was introduced into an E. coli recA441 lexA51 strain which constitutively expresses SOS functions. Weigle mutagenesis of phage lambda could occur in the resulting strain in the absence of host cell irradiation, and was increased when the recA441 (tif) allele was activated by increased temperature and excess adenine. The inability of recF strains to support Weigle mutagenesis can therefore be ascribed to a defect in expression of SOS functions after irradiation.
大肠杆菌recF突变体对紫外线照射的λ噬菌体进行韦格勒诱变的能力大大降低。将recF 332::Tn3突变引入到组成型表达SOS功能的大肠杆菌recA441 lexA51菌株菌株中。在所得菌株中，即使没有宿主细胞照射，噬菌体λ的韦格勒诱变也能发生，并且当recA441（tif）等位基因通过升高温度和添加过量腺嘌呤而被激活时，诱变作用增强。因此，recF菌株无法支持韦格勒诱变可归因于照射后SOS功能表达的缺陷。
