School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Heping District, Tianjin 30070, China.
Department of Pathogen biology, School of Basic Medical Science, Tianjin Medical University, 12 Observatory Road, Heping District, Tianjin 30070, China.
Mater Sci Eng C Mater Biol Appl. 2018 Nov 1;92:206-215. doi: 10.1016/j.msec.2018.05.055. Epub 2018 Jun 23.
Surface modification of titanium with a hydroxyapatite (HAP) coating can improve the bioactivity of pristine titanium. The traditional techniques for coating HAP on titanium involve nonmild treatments using strong bases or acids or high temperatures. In this study, the coating of HAP was carried out by a novel methodology called phase-transited lysozyme-assisted hydroxyapatite formation (PAH); in this process of biomimetic mineralization, the abundant functional carboxyl groups of phase-transited lysozyme (PTL) were responsible for the nucleation of HAP crystals by concentrating Ca ions at the interface between PTL and CaCl solution and for the subsequent growth of HAP crystals occurring in simulated body fluid (SBF). In vitro and in vivo experiments verified that the surface of titanium modified with the HAP/PTL-Ti multilayer was endowed with improved biocompatibility and osteoinductivity compared with those of pristine titanium. Therefore, PAH is a simple, rapid, low-cost and green process for the surface modification of titanium with an HAP coating and thus will be a promising methodology for the surface modification of titanium implants.
通过在钛表面进行羟基磷灰石（HAP）涂层处理可以提高原始钛的生物活性。传统的在钛表面涂层 HAP 的技术涉及使用强碱或强酸或高温的非温和处理。在这项研究中，通过一种称为相转变溶菌酶辅助羟基磷灰石形成（PAH）的新方法进行了 HAP 的涂层处理；在这个仿生矿化过程中，相转变溶菌酶（PTL）中丰富的功能羧基负责通过在 PTL 和 CaCl 溶液之间的界面浓缩 Ca 离子来引发 HAP 晶体的成核，并负责随后在模拟体液（SBF）中发生的 HAP 晶体的生长。体外和体内实验验证了与原始钛相比，经过 HAP/PTL-Ti 多层处理的钛表面具有更好的生物相容性和骨诱导性。因此，PAH 是一种简单、快速、低成本和绿色的钛表面 HAP 涂层处理方法，有望成为钛植入物表面改性的一种有前途的方法。
