DNA介导的一种可使O6-甲基鸟嘌呤去甲基化的人类DNA修复基因的转移与表达。
DNA-mediated transfer and expression of a human DNA repair gene that demethylates O6-methylguanine.
作者信息
Ding R, Ghosh K, Eastman A, Bresnick E
出版信息
Mol Cell Biol. 1985 Nov;5(11):3293-6. doi: 10.1128/mcb.5.11.3293-3296.1985.
Abstract
Human liver DNA was transfected into CHO cells (mex-) along with pSV2gpt and colonies were selected first for resistance to mycophenolic acid and then to chloroethylnitrosourea. Transformants were obtained that contained approximately 10,000 molecules of O6-alkylguanine alkyltransferase (mex+) per cell. Their genome contained at least three copies of the human Alu sequence.
摘要
将人类肝脏DNA与pSV2gpt一起转染到CHO细胞（mex-）中，首先选择对霉酚酸有抗性的菌落，然后选择对氯乙基亚硝脲有抗性的菌落。获得了每个细胞含有约10,000个O6-烷基鸟嘌呤烷基转移酶分子（mex+）的转化体。它们的基因组至少包含三个拷贝的人类Alu序列。
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