Knight Daniel B, Rudin Susan D, Bonomo Robert A, Rather Philip N
Research Service, Atlanta Veterans Affairs Medical Center, Decatur, GA, United States.
Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH, United States.
Front Microbiol. 2018 Aug 21;9:1902. doi: 10.3389/fmicb.2018.01902. eCollection 2018.
is a member of the (ACB) complex. Increasingly, reports are emerging of the pathogenic profile and multidrug resistance (MDR) phenotype of this species. To define novel therapies to overcome resistance, we queried the role of the major efflux pumps in strain M2 on antimicrobial susceptibility profiles. strains with the following mutations were engineered by allelic replacement; Δ, Δ, and Δ. In these isogenic strains, we show that the Δ mutation increased susceptibility to beta-lactams, beta-lactam/beta-lactamase inhibitors, chloramphenicol, monobactam, tigecycline, and trimethoprim. The Δ mutation had a minor effect on resistance to certain beta-lactams, rifampicin and tigecycline. In addition, the Δ mutation resulted in a significant decrease in surface motility and a minor decrease in biofilm formation. Our results indicate that the efflux pump, AdeIJK, has additional roles outside of antibiotic resistance in .
是（ACB）复合体的成员。关于该物种的致病特征和多重耐药（MDR）表型的报道越来越多。为了确定克服耐药性的新疗法，我们探究了主要外排泵在菌株M2对抗菌药物敏感性谱中的作用。通过等位基因替换构建了具有以下突变的菌株；Δ、Δ和Δ。在这些同基因菌株中，我们表明Δ突变增加了对β-内酰胺类、β-内酰胺/β-内酰胺酶抑制剂、氯霉素、单环β-内酰胺类、替加环素和甲氧苄啶的敏感性。Δ突变对某些β-内酰胺类、利福平和替加环素的耐药性有轻微影响。此外，Δ突变导致表面运动性显著降低，生物膜形成略有减少。我们的结果表明，外排泵AdeIJK在除抗生素耐药性之外在中还有其他作用。
