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用于设计两种等温扩增检测方法以检测冷季型草坪草根部感染真菌的数据。

Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses.

作者信息

Karakkat Brijesh B, Hockemeyer Kurt, Franchett Margot, Olson Megan, Mullenberg Cortney, Koch Paul L

机构信息

Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI, USA.

出版信息

Data Brief. 2018 Aug 17;20:471-479. doi: 10.1016/j.dib.2018.08.021. eCollection 2018 Oct.

DOI:10.1016/j.dib.2018.08.021
PMID:30186897
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6122334/
Abstract

Loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are two rapid isothermal amplification methods for detecting three common fungal root pathogens of cool-season turfgrass: , and Detection of root-infecting fungi on cool-season turfgrasses using loop-mediated isothermal amplification and recombinase polymerase amplification (Karakkat et al., 2018) [1]. The data provided here describe the information for designing primers and probes for LAMP and RPA, how specific they are for each of the three fungi, and the evaluation of RPA on field samples.

摘要

环介导等温扩增（LAMP）和重组酶聚合酶扩增（RPA）是两种用于检测冷季型草坪草三种常见根部真菌病原体的快速等温扩增方法： 、 和 。利用环介导等温扩增和重组酶聚合酶扩增检测冷季型草坪草上的根部感染真菌（卡拉卡特等人，2018年）[1]。此处提供的数据描述了用于LAMP和RPA的引物和探针设计信息、它们对三种真菌中每种真菌的特异性以及RPA在田间样本上的评估。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b68/6122334/0b673575c421/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b68/6122334/f265abe090dc/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b68/6122334/0dcfeb421247/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b68/6122334/b1d80d3f6f5b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b68/6122334/0b673575c421/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b68/6122334/f265abe090dc/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b68/6122334/0dcfeb421247/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b68/6122334/b1d80d3f6f5b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b68/6122334/0b673575c421/gr4.jpg

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本文引用的文献

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First Report of Magnaporthe poae, Cause of Summer Patch Disease on Annual Bluegrass, in Canada.加拿大一年生早熟禾夏季斑病病原菌——早熟禾炭疽菌的首次报道
Plant Dis. 2012 Nov;96(11):1698. doi: 10.1094/PDIS-06-12-0530-PDN.
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Detection of root-infecting fungi on cool-season turfgrasses using loop-mediated isothermal amplification and recombinase polymerase amplification.使用环介导等温扩增法和重组酶聚合酶扩增法检测冷季型草坪草上的根部感染真菌。
J Microbiol Methods. 2018 Aug;151:90-98. doi: 10.1016/j.mimet.2018.06.011. Epub 2018 Jun 28.
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