Paul S, Said S I
Peptides. 1986;7 Suppl 1:147-9. doi: 10.1016/0196-9781(86)90177-4.
The zwitterionic detergent 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate (CHAPS) was used to solubilize functional receptors for vasoactive intestinal peptide (VIP) from guinea pig lung. The binding of [125I]-VIP to the soluble receptors was time-dependent, reversible and saturable. Trypsin destroyed the activity of the receptors, indicating their proteinic nature. Dithiothreitol reduced the binding in a dose-dependent manner, suggesting the importance of disulfide bond(s) in receptor-binding activity. Binding of [125I]-VIP to the receptors was reversible and was competitively inhibited by unlabeled VIP, with 50% inhibition obtained at 305 nM VIP.
两性离子去污剂3-[(3-胆酰胺丙基)二甲基铵]-1-丙烷磺酸盐（CHAPS）用于溶解豚鼠肺中血管活性肠肽（VIP）的功能性受体。[125I]-VIP与可溶性受体的结合具有时间依赖性、可逆性和饱和性。胰蛋白酶破坏了受体的活性，表明其蛋白质性质。二硫苏糖醇以剂量依赖性方式降低结合，提示二硫键在受体结合活性中的重要性。[125I]-VIP与受体的结合是可逆的，并被未标记的VIP竞争性抑制，在305 nM VIP时获得50%的抑制率。
