Roman Dongang Nana Rodrigue, Anne Ngono Ngane Rosalie, Singh Vineeta, Luther Koanga Mogtomo Martin, Chantal Ngonde Essome Marie, Albert Mouelle Sone
Department of Biochemistry, Faculty of Sciences, University of Douala, P.O Box 24157, Douala, Cameroon.
Institute of Medical Research and Medicinal Plants Studies (IMPM), P.O Box 13033, Yaoundé, Cameroon.
Heliyon. 2018 Aug 30;4(8):e00760. doi: 10.1016/j.heliyon.2018.e00760. eCollection 2018 Aug.
In this cross-sectional study, we investigated host genetic factors and ethnic variation in circulating merozoite surface protein 2 (msp-2) clones among children with asymptomatic malaria. Isolates from seventy two asymptomatic malaria children were used for genotyping block 3 of msp-2 gene by nested polymerase chain reaction (PCR). Sickle cell trait and glucose-6-phosphate dehydrogenase (G6PD) deficiency were analysed by restriction fragment length polymorphism of DNA products from PCR targeting codons 6 and 68 of the beta-globin (HBB) and G6PD genes respectively. ABO blood group was typed by agglutination method. A total of forty two msp-2 genotypes (20 for 3D7 and 22 for FC27) were detected for an average (standard error of mean) multiplicity of infection (MOI) of 2.45 (0.16). The MOI was statistically the same among the five identified ethnic groups (P = 0.83). The overall prevalence of sickle cell trait and G6PD deficiency were 12.50 % and 22.22 % respectively. MOI was similar between children with Hb AA and Hb AS genotypes (P = 0.42). MOI was significantly high among children with a mutant G6PD genotype (P = 0.017). MOI was significantly higher in blood group O than group A (P = 0.03). Our findings show that although ethnicity and sickle cell trait have no association with MOI, the association was observed with G6PD genotype and ABO group. The results suggest the need for extension and expansion of the current study in order to investigate the mechanisms involved.
在这项横断面研究中，我们调查了无症状疟疾儿童中宿主遗传因素及循环裂殖子表面蛋白2（msp-2）克隆的种族差异。采用巢式聚合酶链反应（PCR）对72例无症状疟疾儿童的分离株进行msp-2基因第3区基因分型。分别通过针对β-珠蛋白（HBB）基因密码子6和68以及葡萄糖-6-磷酸脱氢酶（G6PD）基因的PCR产物的限制性片段长度多态性分析镰状细胞性状和葡萄糖-6-磷酸脱氢酶（G6PD）缺乏症。采用凝集法进行ABO血型分型。共检测到42种msp-2基因型（3D7型20种，FC27型22种），平均感染复数（MOI）（平均标准误）为2.45（0.16）。在五个已识别的种族群体中，MOI在统计学上相同（P = 0.83）。镰状细胞性状和G6PD缺乏症的总体患病率分别为12.50%和22.22%。Hb AA和Hb AS基因型儿童的MOI相似（P = 0.42）。突变型G6PD基因型儿童的MOI显著较高（P = 0.017）。O血型儿童的MOI显著高于A血型儿童（P = 0.03）。我们的研究结果表明，虽然种族和镰状细胞性状与MOI无关，但与G6PD基因型和ABO血型有关。结果表明需要扩大和延伸当前研究以探究其中涉及的机制。
