一种研究小鼠表皮生长因子分子结构与功能关系的合成方法。
A synthetic approach to structure-function relationships in the murine epidermal growth factor molecule.
作者信息
Heath W F, Merrifield R B
出版信息
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6367-71. doi: 10.1073/pnas.83.17.6367.
Abstract
Murine epidermal growth factor, a 53-amino acid peptide that is mitogenic for a number of cell types, has been synthesized by the solid-phase method. The synthetic peptide is identical to the natural material in amino acid composition, chromatographic behavior, receptor binding, and stimulation of DNA synthesis. Fragments of the EGF molecule corresponding to residues 42-53, 32-53, and 15-53 were constructed as well as the methionine sulfoxide derivative of EGF, [Met(O)21]EGF-(1-53), and a polymeric form of EGF. [Met(O)21]EGF-(1-53) was slightly less active than EGF in receptor binding and stimulation of DNA synthesis. Polymeric EGF was 1/100th as active as EGF, while EGF-(15-53) was less potent than EGF-(1-53) by a factor of 10(4). EGF-(32-53) was even less active and EGF-(43-53) was inactive.
摘要
小鼠表皮生长因子是一种由53个氨基酸组成的肽，对多种细胞类型具有促有丝分裂作用，已通过固相法合成。合成肽在氨基酸组成、色谱行为、受体结合及刺激DNA合成方面与天然物质相同。构建了对应于42 - 53、32 - 53和15 - 53位残基的表皮生长因子（EGF）分子片段，以及EGF的甲硫氨酸亚砜衍生物[Met(O)21]EGF-(1 - 53)和一种聚合形式的EGF。[Met(O)21]EGF-(1 - 53)在受体结合和刺激DNA合成方面的活性略低于EGF。聚合EGF的活性仅为EGF的1/100，而EGF-(15 - 53)的效力比EGF-(1 - 53)低10⁴倍。EGF-(32 - 53)的活性更低，而EGF-(43 - 53)无活性。
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