College of Biology, Hunan University, Changsha, Hunan, 410082, China.
State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, Hunan, 410082, China.
Macromol Biosci. 2018 Nov;18(11):e1800205. doi: 10.1002/mabi.201800205. Epub 2018 Sep 5.
The development of accurate and predictive in vitro experimental models of human tumors consistent with in vivo tumor microenvironments has garnered great attention in modern cancer research. 3D scaffolds are fabricated in this study by E-jet 3D printing with the aim of replicating the functionalities of tumor microenvironments in vitro which could be applicable as screening platforms for novel therapeutic strategies. Tumor protein 53 (p53) plays an important role in penetration and migration in 2D cell culture. However, whether or not p53 has the same function in 3D cell culture and the underlying mechanisms are poorly understood. Results show that p53 deletion significantly decreases the speed of migration and proliferation of cancer cells within 3D environments. This study unveils aspects of cancer cell motility and migration and the steps involved in subsequent cancer metastases, which provides a new perspective and platform for the research of tumor metastasis therapy.
在肿瘤研究中，开发与体内肿瘤微环境一致的精确、可预测的人类肿瘤体外实验模型受到了广泛关注。本研究采用 E-jet 3D 打印技术制造了 3D 支架，旨在体外复制肿瘤微环境的功能，可作为新型治疗策略的筛选平台。肿瘤蛋白 53（p53）在 2D 细胞培养中的穿透和迁移中起着重要作用。然而，p53 是否在 3D 细胞培养中具有相同的功能以及其潜在机制尚不清楚。研究结果表明，p53 缺失会显著降低癌细胞在 3D 环境中的迁移和增殖速度。本研究揭示了癌细胞迁移和运动的一些方面以及随后癌症转移涉及的步骤，为肿瘤转移治疗的研究提供了新的视角和平台。
