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克什米尔山谷纳拉赫苏克纳格地区感染*Pomphorhynchus*的*Schizothorax plagiostomus*体内谷胱甘肽-S-转移酶、超氧化物歧化酶(GST、SOD)水平、蛋白质含量及脂质过氧化情况

Glutathione-S-transferase, Superoxide Dismutase (GST, SOD) Levels, Protein Content and Lipid Peroxidation in <I>Schizothorax plagiostomus </I>under the Infection of <I>Pomphorhynchus </I>in Nallah Sukhnag of Kashmir Valley.

作者信息

Nabi Shafaquat, Tanveer Syed, Ganie Showkat Ahmad

出版信息

Pak J Biol Sci. 2017;20(9):442-446. doi: 10.3923/pjbs.2017.442.446.

Abstract

BACKGROUND AND OBJECTIVE

Presence of parasites in fishes has been reported to severely damage the status of antioxidants in them. Among helminth parasites, Pomphorhyncus an acanthocephalan parasite is commonly found in Schizothorax fish of Kashmir and is causing a considerable damage to fish health. The aim of this study was to investigate the antioxidant status in muscle, intestine and liver tissues of Schizothorax plagiostomus parasitized by Pomphorhyncus.

MATERIALS AND METHODS

For this, 9 fish specimens collected from Nallah Sukhnag (Budgam) were found to be infected only with Pomphorhyncus, an acanthocephalan parasite. Fishes having the infection of Pomphorhynchus and the same number of uninfected ones were then analyzed for enzymatic antioxidants. Lipid peroxidation and protein content was also assayed for both infected and uninfected fishes. Statistically the whole data were represented as mean and standard deviation.

RESULTS

RESULTS showed the reduction of glutathione-s-transferase (GST), superoxide dismutase (SOD), protein content while increase in lipid peroxidation in infected muscle, intestine and liver tissues as compared to respective organs of the uninfected fishes.

CONCLUSION

This study concluded that parasitic infections induce oxidative stress.

摘要

背景与目的

据报道,鱼类体内寄生虫的存在会严重损害其抗氧化状态。在蠕虫寄生虫中,棘头虫寄生虫Pomphorhyncus常见于克什米尔的裂腹鱼中,对鱼类健康造成了相当大的损害。本研究的目的是调查被Pomphorhyncus寄生的高原裸裂尻鱼肌肉、肠道和肝脏组织中的抗氧化状态。

材料与方法

为此,从纳勒苏克纳格(布德加姆)采集的9个鱼类标本被发现仅感染了棘头虫寄生虫Pomphorhyncus。然后对感染了Pomphorhynchus的鱼和相同数量的未感染鱼进行酶促抗氧化剂分析。还对感染和未感染的鱼进行了脂质过氧化和蛋白质含量测定。从统计学角度,所有数据均以平均值和标准差表示。

结果

结果显示,与未感染鱼相应器官相比,感染鱼的肌肉、肠道和肝脏组织中谷胱甘肽 - S - 转移酶(GST)、超氧化物歧化酶(SOD)、蛋白质含量降低,脂质过氧化增加。

结论

本研究得出结论,寄生虫感染会诱导氧化应激。

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