Li Xuewen, Makavitskaya Maryia, Samokhina Veranika, Mackievic Viera, Navaselsky Ilya, Hryvusevich Palina, Smolikova Galina, Medvedev Sergei, Shabala Sergey, Yu Min, Demidchik Vadim
a Department of Horticulture , Foshan University , Foshan , China.
b Department of Plant Cell Biology and Bioengineering, Biological Faculty , Belarusian State University , Minsk , Belarus.
Plant Signal Behav. 2018;13(9):e1514895. doi: 10.1080/15592324.2018.1514895. Epub 2018 Sep 6.
Functions of exogenous L-ascorbic acid in plant roots are poorly understood. Recent study by Makavitskaya et al. (doi.org/10.1093/jxb/ery056) has demonstrated that exogenous ascorbate can be released from roots in response to salt stress, and can trigger elevation in the cytosolic free Ca. Here, we report that exogenous ascorbate significantly modifies root elongation in Arabidopsis thaliana. Using a medium exchange technique, we have shown that 10-100 µM ascorbate induces small but significant increase in root elongation while higher levels cause its dramatic decrease. Root border cells of Pisum sativum have been losing viability twice faster in the presence of ascorbate that under control conditions, as tested by the confocal microscopy and a combined staining with propidium iodide and fluorescein diacetate.
外源L-抗坏血酸在植物根系中的功能尚不清楚。Makavitskaya等人最近的研究（doi.org/10.1093/jxb/ery056）表明，外源抗坏血酸盐可在盐胁迫下从根系中释放出来，并能引发胞质游离钙升高。在此，我们报告外源抗坏血酸盐显著改变了拟南芥的根伸长。使用培养基交换技术，我们发现10-100µM抗坏血酸盐可诱导根伸长出现小幅但显著的增加，而更高水平则会导致根伸长急剧下降。通过共聚焦显微镜以及碘化丙啶和荧光素二乙酸的联合染色测试发现，在抗坏血酸存在的情况下，豌豆的根边缘细胞失去活力的速度比对照条件下快两倍。
