Institute for Chemical and Bioengineering, ETH Zürich, Zürich, Switzerland.
Clinic of Preventive Dentistry, Periodontology and Cariology, University of Zürich, Center of Dental Medicine, Zürich, Switzerland.
J Biomed Mater Res B Appl Biomater. 2019 May;107(4):1180-1188. doi: 10.1002/jbm.b.34211. Epub 2018 Sep 6.
Silicone is an important material family used for various medical implants. It is biocompatible, but its bioinertness prevents cell attachment, and thus tissue biointegration of silicone implants. This often results in constrictive fibrosis and implant failure. Bioglass 45S5® (BG) could be a suitable material to alter the properties of silicone, render it bioactive and improve tissue integration. Therefore, BG micro- or nanoparticles were blended into medical-grade silicone and 2D as well as 3D structures of the resulting composites were analyzed in ovo by a chick chorioallantoic membrane (CAM) assay. The biomechanical properties of the composites were measured and the bioactivity of the composites was verified in simulated body fluid. The bioactivity of BG-containing composites was confirmed visually by the formation of hydroxyapatite through scanning electron microscopy as well as by infrared spectroscopy. BG stiffens as prepared non-porous composites by 13% and 36% for micro- and nanocomposites respectively. In particular, after implantation for 7 days, the Young's modulus had increased significantly from 1.20 ± 0.01 to 1.57 ± 0.03 MPa for microcomposites and 1.44 ± 0.03 to 1.69 ± 0.29 MPa to for nanocpmosites. Still, the materials remain highly elastic and are comparably soft. The incorporation of BG into silicone overcame the bioinertness of the pure polymer. Although the overall tissue integration was weak, it was significantly improved for BG-containing porous silicones (+72% for microcomposites) and even further enhanced for composites containing nanoparticles (+94%). These findings make BG a suitable material to improve silicone implant properties. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1180-1188, 2019.
硅酮是一种用于各种医疗植入物的重要材料家族。它是生物相容的，但它的生物惰性阻止了细胞附着，从而导致硅酮植入物的组织生物整合。这通常导致紧缩性纤维化和植入物失败。生物玻璃 45S5®（BG）可能是一种改变硅酮性质、使其具有生物活性并改善组织整合的合适材料。因此，BG 微/纳米颗粒被混入医用级硅酮中，并通过鸡绒毛尿囊膜（CAM）试验在鸡胚中分析所得复合材料的 2D 和 3D 结构。复合材料的生物力学性能进行了测量，并在模拟体液中验证了复合材料的生物活性。通过扫描电子显微镜以及红外光谱观察到 BG 含有复合材料形成羟基磷灰石，从而证实了 BG 含有复合材料的生物活性。BG 使未制备多孔复合材料分别增加 13%和 36%的刚性，微复合材料和纳米复合材料。特别是，植入 7 天后，微复合材料的杨氏模量从 1.20±0.01 显著增加到 1.57±0.03 MPa，纳米复合材料从 1.44±0.03 增加到 1.69±0.29 MPa。尽管如此，这些材料仍然具有高度弹性且相对柔软。将 BG 掺入硅酮中克服了纯聚合物的生物惰性。尽管整体组织整合较弱，但对于含有 BG 的多孔硅酮（微复合材料增加 72%）甚至对于含有纳米颗粒的复合材料（增加 94%），组织整合得到了显著改善。这些发现使 BG 成为改善硅酮植入物性能的合适材料。©2018 年威利父子公司。J 生物医学材料研究部分 B：应用生物材料 107B：1180-1188，2019 年。
