National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhejiang, Zhoushan 316004, China.
National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhejiang, Zhoushan 316004, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2018 Dec;214:43-51. doi: 10.1016/j.cbpc.2018.08.008. Epub 2018 Sep 3.
Estrogen could lead to abnormal modulation or disruption of physical development, reproduction and sexual behavior in aquatic wildlife, especially in fish. Information on the toxicity of estrogens to native species in that can be used in site-specific risk assessments is scarce. In the present study, one zona pellucida 3 (ZP3) homologue termed CaZP3 was firstly identified from topmouth culter Culter alburnus, following its structural characteristics, tissue distribution and transcriptional modulation to 17α-ethinylestradiol (EE2) exposure were investigated. Meanwhile, vitellogenin (VTG) gene was employed to provide a comparison of the reactive ability to EE2 induction. The CaZP3 characterized with analogical functional domains such as ZP domain, SP, IHP, EHP, 12 cysteine residues, one N-linked glycosylation site and two conserved O-linked glycosylation sites and equal number of eight exons and seven introns with ZP3 counterparts of higher species. CaZP3 mRNA predominantly expressed in ovary, besides, highly expressed in female heart and male muscle and relatively high expressed in testis. CaZP3 has the lower reactive ability to EE2 induction in comparison with VTG, however, CaZP3 transcripts were significantly induced in gonads of both male and female culter by EE2 and could be used as an alternative biomarker to monitor EE2 activity. The present results supplement the database for toxicity of EE2, especially for fish species endemic to China and provide some useful information for the monitoring of EE2 activity in aquatic environment.
雌激素可能导致水生野生动物，尤其是鱼类的身体发育、繁殖和性行为出现异常调节或中断。有关雌激素对该地区本地物种毒性的信息可用于特定地点的风险评估，但非常有限。在本研究中，首次从翘嘴红鲌（Culter alburnus）中鉴定出一种称为 CaZP3 的透明带 3（ZP3）同源物，随后研究了其结构特征、组织分布以及对 17α-乙炔基雌二醇（EE2）暴露的转录调节。同时，还利用卵黄蛋白原（VTG）基因提供与 EE2 诱导反应能力的比较。CaZP3 具有类似的功能域，如 ZP 域、SP、IHP、EHP、12 个半胱氨酸残基、一个 N-连接糖基化位点和两个保守的 O-连接糖基化位点，以及与高等物种 ZP3 对应的八个外显子和七个内含子。CaZP3 mRNA 主要在卵巢中表达，此外，在雌性心脏和雄性肌肉中高度表达，在睾丸中相对高表达。与 VTG 相比，CaZP3 对 EE2 诱导的反应能力较低，但 EE2 可显著诱导雌雄翘嘴红鲌性腺中 CaZP3 转录本的表达，可作为监测 EE2 活性的替代生物标志物。本研究结果补充了 EE2 毒性的数据库，特别是对中国特有的鱼类物种，为监测水生环境中 EE2 活性提供了一些有用信息。
