Cao W, Liu X, Xu X, Zeng M, Sun B, Yu X, Wang N, Mao H, Zhang B, Yuan Y, Xing C
Department of Nephrology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Lupus. 2018 Nov;27(13):2014-2019. doi: 10.1177/0961203318796295. Epub 2018 Sep 6.
The Src homology and collagen A (ShcA) adaptor protein that binds to tyrosine kinase receptors. ShcA plays a role in insulin signaling, stress resistance and energy metabolism. The 66-kDa Src homology 2 domain-containing protein (p66shc) belongs to the ShcA family and has been associated with reactive oxygen species (ROS); increased ROS is involved in the pathology of lupus nephritis (LN). However, whether ShcA can act as a biomarker for oxidative injury in LN is unknown. This study is aimed to investigate the ShcA expression in kidney tissues from patients presenting with LN and the association between ShcA expression and clinical parameters. Renal biopsy tissues were obtained from 62 LN, 20 primary membranous nephropathy (MN) and 10 other secondary MN patients. ShcA was measured by immunofluorescence. The expression of ShcA in the membranous lupus nephritis (class V) group showed a higher trend but there were no significant differences compared with pure mesangial disease (class II) and proliferative (Class III/IV) lupus nephritis. ShcA deposits were negative in primary and other secondary MN. ShcA might act as a new biomarker and a diagnostic tool to identify membranous lupus nephritis with other MN.
Src同源和胶原蛋白A（ShcA）衔接蛋白可与酪氨酸激酶受体结合。ShcA在胰岛素信号传导、应激抵抗和能量代谢中发挥作用。含66 kDa Src同源2结构域的蛋白（p66shc）属于ShcA家族，且与活性氧（ROS）有关；ROS增加参与狼疮性肾炎（LN）的病理过程。然而，ShcA是否可作为LN氧化损伤的生物标志物尚不清楚。本研究旨在调查LN患者肾组织中ShcA的表达情况以及ShcA表达与临床参数之间的关联。从62例LN患者、20例原发性膜性肾病（MN）患者和10例其他继发性MN患者获取肾活检组织。通过免疫荧光法检测ShcA。膜性狼疮性肾炎（V类）组中ShcA的表达呈较高趋势，但与单纯系膜疾病（II类）和增殖性（III/IV类）狼疮性肾炎相比无显著差异。原发性和其他继发性MN中ShcA沉积物为阴性。ShcA可能作为一种新的生物标志物和诊断工具，用于鉴别膜性狼疮性肾炎与其他MN。
