Centre for Infectious Disease Control, RIVM, Bilthoven, The Netherlands.
Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands.
Sci Rep. 2018 Sep 6;8(1):13337. doi: 10.1038/s41598-018-31630-z.
The surface proteins of the mumps virus, the fusion protein (F) and haemagglutinin-neuraminidase (HN), are key factors in mumps pathogenesis and are important targets for the immune response during mumps virus infection. We compared the predicted amino acid sequences of the F and HN genes from Dutch mumps virus samples from the pre-vaccine era (1957-1982) with mumps virus genotype G strains (from 2004 onwards). Genotype G is the most frequently detected mumps genotype in recent outbreaks in vaccinated communities, especially in Western Europe, the USA and Japan. Amino acid differences between the Jeryl Lynn vaccine strains (genotype A) and genotype G strains were predominantly located in known B-cell epitopes and in N-linked glycosylation sites on the HN protein. There were eight variable amino acid positions specific to genotype A or genotype G sequences in five known B-cell epitopes of the HN protein. These differences may account for the reported antigenic differences between Jeryl Lynn and genotype G strains. We also found amino acid differences in and near sites on the HN protein that have been reported to play a role in mumps virus pathogenesis. These differences may contribute to the occurrence of genotype G outbreaks in vaccinated communities.
腮腺炎病毒的表面蛋白，融合蛋白（F）和血凝素神经氨酸酶（HN），是腮腺炎发病机制中的关键因素，也是腮腺炎病毒感染期间免疫反应的重要靶点。我们比较了来自疫苗接种前时代（1957-1982 年）的荷兰腮腺炎病毒样本的 F 和 HN 基因的预测氨基酸序列与基因型 G 株（自 2004 年以来）。基因型 G 是最近在接种疫苗的社区中爆发的腮腺炎的最常见基因型，尤其是在西欧、美国和日本。Jeryl Lynn 疫苗株（基因型 A）与基因型 G 株之间的氨基酸差异主要位于已知的 B 细胞表位和 HN 蛋白上的 N-连接糖基化位点。在 HN 蛋白的五个已知 B 细胞表位中，有八个特定于基因型 A 或基因型 G 序列的可变氨基酸位置。这些差异可能解释了 Jeryl Lynn 株与基因型 G 株之间报道的抗原差异。我们还发现了 HN 蛋白上和附近的氨基酸差异，这些差异已被报道在腮腺炎病毒发病机制中起作用。这些差异可能导致接种疫苗社区中基因型 G 爆发的发生。
