Genetic diversity at the C-terminal domain of knob-associated histidine-rich protein (KAHRP) of isolates from Burundi, Eastern Africa.

The knob-associated histidine-rich protein (KAHRP) is an exported parasite protein and the major component of infected erythrocytes by . histidine-rich protein-1 (PfHRP-1) is docked by KAHRP, which this interaction plays a significant role in cytoadherence of the malaria protozoan to erythrocytes and pathogenicity. The most polymorphic region of the PfHRP-1 is the C-terminal of decapeptide repeat domain (region III). The main objective of this study was to explore the genetic diversity at the region III of KAHRP in isolates from Burundi. In the present study, the nested PCR was performed for the amplification of the coding gene ( gene) for III in 35 isolates from Burundi. The nested PCR products of seven randomly selected isolates were purified and then sequenced. As the result, three allelic forms (340 bp, 370 bp, and 400 bp) were seen at the C-terminal domain of gene. The existence of multiple alleles of the gene revealed the presence of different strains in Burundi. It is suggested that the results could be useful in designing and the improvement of targeted therapy agents for malaria.