Carver F J, Meints R H
J Exp Zool. 1977 Jul;201(1):37-46. doi: 10.1002/jez.1402010105.
A new in vitro technique has been described for demonstrating the presence of an erythropoietic factor in the circulating blood of frogs. The assay system consisted of MC33 medium, erythropoietically active spleen cells from Rana pipiens, and plasma or serum from frogs made anemic via phenylhydrazine or bleeding. The spleen cells, which remain erythropoietically active for up to nine days, were found to incorporate 59Fe, [3H]thymidine, [3H]uridine, and [3H]leucine at a greater rate in the presence of plasma or serum from anemic versus normal frogs. The hormones triiodothyronine, prolactin, and erythropoietin were not effective in eliciting an hemopoietic response. The data presented suggest that the spleen from that adult frog is a major site of erythroid differentiation and maturation.
一种新的体外技术已被描述用于证明青蛙循环血液中存在促红细胞生成因子。该检测系统由MC33培养基、来自豹蛙的具有促红细胞生成活性的脾细胞以及通过苯肼或放血导致贫血的青蛙的血浆或血清组成。发现脾细胞在贫血青蛙与正常青蛙的血浆或血清存在下，能以更高的速率掺入59Fe、[3H]胸腺嘧啶核苷、[3H]尿苷和[3H]亮氨酸，且其促红细胞生成活性可持续长达九天。三碘甲状腺原氨酸、催乳素和促红细胞生成素等激素在引发造血反应方面无效。所呈现的数据表明，成年青蛙的脾脏是红细胞分化和成熟的主要部位。
