Institute of Agro-food Science & Technology , Guangxi Academy of Agricultural Sciences , 174 East Daxue Road , Nanning 530007 , China.
Guangxi Crop Genetic Improvement Laboratory , Nanning 530007 , China.
J Agric Food Chem. 2018 Oct 10;66(40):10628-10639. doi: 10.1021/acs.jafc.8b01844. Epub 2018 Sep 28.
The roles of microRNAs (miRNAs) related to ethylene response in banana fruits remain unknown because many miRNAs are differentially expressed as the fruit ripens, making the identification of ethylene-responsive miRNAs difficult. Using newly harvested banana fruits (within 5 h after harvest) as material, we found that these fruit did not ripen when treated with 5 μL/L of ethylene for 12 h at 22 °C. Two miRNA libraries were generated from newly harvested banana fruits with and without ethylene treatment and sequenced. In total, 128 known miRNAs belonging to 42 miRNA families were obtained, and 12 novel miRNAs were identified. Among them, 22 were differentially expressed in response to ethylene treatment, among which 6 known miRNAs and their putative targets were validated using qRT-PCR. These putative targets encoded proteins including GATA, ARF, DLC, and AGO, etc. KEGG and GO analyses showed that miRNAs differentially expressed in response to ethylene mainly function in the molecular and biological processes.
乙烯反应相关 microRNAs（miRNAs）在香蕉果实中的作用尚不清楚，因为许多 miRNAs 在果实成熟过程中呈现差异表达，这使得乙烯反应性 miRNAs 的鉴定变得困难。本研究使用刚收获的香蕉果实（收获后 5 小时内）作为材料，发现这些果实经 5 μL/L 乙烯处理 12 小时、22°C 下不会成熟。从乙烯处理和未处理的刚收获的香蕉果实中分别生成两个 miRNA 文库并进行测序。共获得 128 个属于 42 个 miRNA 家族的已知 miRNAs，并鉴定出 12 个新的 miRNAs。其中，有 22 个 miRNA 对乙烯处理有差异表达，其中 6 个已知 miRNA 及其假定靶标通过 qRT-PCR 进行了验证。这些假定靶标编码的蛋白质包括 GATA、ARF、DLC 和 AGO 等。KEGG 和 GO 分析表明，对乙烯反应差异表达的 miRNAs 主要在分子和生物过程中发挥作用。
