尼罗罗非鱼肝转录组对环境浓度邻苯二甲酸二（2-乙基己基）酯的响应。
Transcriptomic responses of Nile tilapia (Oreochromis niloticus) liver to environmental concentration of di(2-ethylhexyl)phthalate.
机构信息
Scientific Observing and Experimental Station of South China Sea Fishery Resources & Environments, Ministry of Agriculture, Guangzhou 510300, PR China; Guangdong Provincial Key Lab. of Fishery Ecology and Environment, Guangzhou 510300, PR China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, Guangdong 518060, PR China.
出版信息
Ecotoxicol Environ Saf. 2018 Dec 15;165:70-77. doi: 10.1016/j.ecoenv.2018.08.100. Epub 2018 Sep 4.
Di-(2-ethylhexyl) phthalate (DEHP) is currently the most frequently detected phthalic acid esters (PAEs) compounds and can induce diverse toxicities on aquatic organisms. To understand the molecular responses of fish to DEHP, we performed transcriptomic profiles in liver of tilapia (Oreochromis niloticus) which were exposed to environmental concentration of DEHP. A total of 30.10 Mb and 30.16 Mb clean reads were retrieved from the control and DEHP treated libraries, respectively. De-novo assembly of all the clean reads obtained 58,585 unigenes. After comparing the two libraries, 2814 and 1790 genes were identified as significantly increased and depressed, respectively. Gene ontology (GO) classification system and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis demonstrated that DEHP significantly disturbed the expression level of genes associated with immunity, endocrine and reproductive system, lipid metabolism and so on. Quantitative real-time PCR was performed to validate the results of RNA-sequencing (RNA-seq) analysis. The resulting data provide new insights for exploring the molecular basis of tilapia response to DEHP exposure.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是目前最常检测到的邻苯二甲酸酯（PAEs）化合物，可对水生生物产生多种毒性。为了了解鱼类对 DEHP 的分子反应，我们对暴露于环境浓度 DEHP 的罗非鱼（Oreochromis niloticus）肝脏进行了转录组谱分析。从对照和 DEHP 处理的文库中分别检索到 30.10 Mb 和 30.16 Mb 的清洁读数。从所有清洁读数中进行从头组装得到了 58585 个非编码 RNA。在比较这两个文库后，分别鉴定出 2814 个和 1790 个基因的表达水平显著增加和降低。基因本体（GO）分类系统和京都基因与基因组百科全书（KEGG）数据库分析表明，DEHP 显著干扰了与免疫、内分泌和生殖系统、脂质代谢等相关的基因的表达水平。实时定量 PCR 用于验证 RNA 测序（RNA-seq）分析的结果。所得数据为探索罗非鱼对 DEHP 暴露的反应的分子基础提供了新的见解。
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