Odarjuk J, Maretzki D, Gross J, Gerber G
Biomed Biochim Acta. 1986;45(5):593-9.
Striatum synaptosomes prepared from adult rats which had been exposed to postnatal hypoxia incorporate 32P-phosphate into phosphatidylinositol-4,5-trisphosphate (PI-4,5-P2) with decreased rate. 32P-incorporation amounted to 57% of the control for PI-4,5-P2 labeling and was slightly diminished for phosphatidic acid and PI-4-P. Exposure to hypoxia of adult rats did not affect inositol phospholipid labeling. The inhibitory effect of dopamine on 32P-phosphate incorporation was reduced only after postnatal hypoxia. 32P-incorporation rates and the dopamine inhibitory effect were not influenced by external calcium. A working hypothesis is suggested for the dopamine action on specific receptors which may be linked to the polyphosphoinositide metabolism and membrane calcium release. The long lasting effects of an early postnatal hypoxia on 32P-incorporation rates into polyphosphoinositides and phosphatidic acid could reflect the role of the proposed dopamine receptor interaction.
从出生后经历过缺氧的成年大鼠制备的纹状体突触体，将32P - 磷酸盐掺入磷脂酰肌醇 - 4,5 - 三磷酸（PI - 4,5 - P2）的速率降低。PI - 4,5 - P2标记的32P掺入量为对照的57%，磷脂酸和PI - 4 - P的掺入量略有减少。成年大鼠暴露于缺氧环境并不影响肌醇磷脂的标记。只有在出生后缺氧后，多巴胺对32P - 磷酸盐掺入的抑制作用才会降低。32P掺入率和多巴胺抑制作用不受细胞外钙的影响。提出了一个关于多巴胺作用于特定受体的工作假设，该受体可能与多磷酸肌醇代谢和膜钙释放有关。出生后早期缺氧对多磷酸肌醇和磷脂酸32P掺入率的长期影响可能反映了所提出的多巴胺受体相互作用的作用。
