Zharkovskiĭ A M
Biull Eksp Biol Med. 1986 Sep;102(9):298-301.
In the studies of 3H-diazepam binding to the rat brain membranes it has been shown that insufficiently high concentrations of free ligand might lead to incorrect determination of Bmax. Thus, free ligand concentration in the range of 0.5 to 16 nM (the most often used ones) and low receptor-protein concentrations (0.08 to 0.12 mg/sample) were far from being saturating and therefore could not be applied for the analysis in Scatchard coordinates. In this case Bmax value would be considerably below the true Bmax value. It has been concluded that for the determination of Bmax of 3H-benzodiazepine binding the range of concentrations used should be at least 0.25 to 64 nM.
在关于3H-地西泮与大鼠脑膜结合的研究中，已表明游离配体浓度不够高可能会导致对Bmax的测定不准确。因此，0.5至16 nM范围内的游离配体浓度（最常用的浓度）以及低受体蛋白浓度（0.08至0.12 mg/样本）远远未达到饱和状态，所以不能用于Scatchard坐标分析。在这种情况下，Bmax值将远低于真实的Bmax值。已得出结论，对于3H-苯二氮䓬结合的Bmax测定，所使用的浓度范围应至少为0.25至64 nM。
