Biotechnology Research Center and Student Research Committee, Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Nutrition Research Center and Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Int J Biol Macromol. 2018 Dec;120(Pt B):2572-2578. doi: 10.1016/j.ijbiomac.2018.09.033. Epub 2018 Sep 6.
This study is concerned with modification of high methoxy pectin (HMP) assisted by nitrogen AC glow discharge plasma. Intrinsic viscosity of plasma treated pectin (PTP) was measured as primary index for structural changes in pectin molecules at different plasma conditions. The intrinsic viscosity of pectin increased within 7 min of exposure to plasma treatment and then remained constant. According to Fourier Transform Infrared Spectrometry (FT-IR) analysis, the intensity of carboxylate peaks increased in PTP samples due to de-esterification of pectin. Moreover, by decreasing the degree of esterification (DE) in PTP samples, the results of FT-IR were confirmed. Based on high performance size-exclusion chromatography (HPSEC) analysis, the molecular weight of PTP reduced. PTP gel had higher storage and loss modulus and shorter linear viscoelastic region. Moreover, X-ray diffraction (XRD) studies indicated an increase of crystallinity in PTP due to the formation of hydrogen bonds. Results revealed that nitrogen AC glow discharge plasma had significant influence on physicochemical and functional properties of pectin. The result of this study showed AC glow discharge plasma has promising potential towards modification of pectin.
本研究关注的是氮 AC 辉光放电等离子体辅助下的高甲氧基果胶（HMP）的修饰。用特性黏度作为果胶分子结构变化的主要指标，来测量等离子体处理后果胶（PTP）的特性黏度。在暴露于等离子体处理 7 分钟内，果胶的特性黏度增加，然后保持不变。根据傅里叶变换红外光谱（FT-IR）分析，由于果胶的去酯化，PTP 样品中羧酸根峰的强度增加。此外，通过降低 PTP 样品中的酯化度（DE），进一步证实了 FT-IR 的结果。基于高效体积排除色谱（HPSEC）分析，PTP 的分子量降低。PTP 凝胶具有更高的储能模量和损耗模量，以及更短的线性黏弹区。此外，X 射线衍射（XRD）研究表明，由于氢键的形成，PTP 的结晶度增加。结果表明，氮 AC 辉光放电等离子体对果胶的物理化学和功能性质有显著影响。本研究结果表明，AC 辉光放电等离子体在果胶的修饰方面具有广阔的应用前景。
