假定的第二信使影响生精小管中的细胞偶联。
Putative second messengers affect cell coupling in the seminiferous tubules.
作者信息
Grassi F, Monaco L, Fratamico G, Dolci S, Iannini E, Conti M, Eusebi F, Stefanini M
出版信息
Cell Biol Int Rep. 1986 Aug;10(8):631-9. doi: 10.1016/0309-1651(86)90140-2.
Junctional transfer of ions in monolayer primary cultures of Sertoli cells and in intact seminiferous tubules from 20 day-old rats has been investigated by using electrophysiological techniques. The electrotonic coupling of both intact tubule and monolayer culture was inhibited by the presence of dibutyryl cyclic AMP (10(-4) M) in the medium. In contrast, 1-oleoyl-2-acetylglycerol (10(-5) M) and 12-O-tetradecanoyl-phorbol-13-acetate (10(-7) M) decreased the junctional resistivity and increased the extent of the coupling in immature tubules. The significance of this modulation of the cell coupling in the seminiferous epithelium is discussed.
运用电生理技术，对20日龄大鼠的支持细胞单层原代培养物以及完整生精小管中的离子连接转运进行了研究。培养基中存在二丁酰环磷酸腺苷（10⁻⁴ M）时，完整小管和单层培养物的电紧张偶联均受到抑制。相反，1-油酰基-2-乙酰甘油（10⁻⁵ M）和12-O-十四酰佛波醇-13-乙酸酯（10⁻⁷ M）可降低未成熟小管的连接电阻，并增加偶联程度。文中讨论了生精上皮中这种细胞偶联调节的意义。
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