Wang Xiaoyang, Xing Yizhan, Li Yuhong
Department of Cell Biology, Third Military Medical University, Chongqing, China.
Department of Developmental and Cell Biology, University of Irvine, Irvine, CA, USA.
Methods Mol Biol. 2018;1842:69-80. doi: 10.1007/978-1-4939-8697-2_5.
Hair follicle stem cells (HFSCs) are epithelial cells that inhabit in the bulge region of hair follicles. They govern development of hair follicle as well as periodically regeneration of hair follicle. Under special condition, they also play roles in homeostasis of skin and other skin appendages. To characterize HFSCs in vitro, HFSCs must be isolated and cultured. In this chapter, we introduce a mechanical method to isolate HFSCs from mouse vibrissa hair follicle, and a modified method to culture isolated HFSCs. We also describe methods to characterize HFSCs, including clone formation assay and chamber graft assay.
毛囊干细胞（HFSCs）是位于毛囊隆突区的上皮细胞。它们掌控着毛囊的发育以及毛囊的周期性再生。在特殊条件下，它们还在皮肤及其他皮肤附属器的稳态维持中发挥作用。为了在体外对毛囊干细胞进行特性分析，必须分离并培养毛囊干细胞。在本章中，我们介绍一种从小鼠触须毛囊中分离毛囊干细胞的机械方法，以及一种改良的培养分离出的毛囊干细胞的方法。我们还描述了对毛囊干细胞进行特性分析的方法，包括克隆形成试验和腔室移植试验。
