Nakajima Y, Ishida M, Kajita Y, Ura Y, Ochi Y, Miyazaki T, Hachiya T, Ijichi H
Endocrinol Jpn. 1986 Jun;33(3):375-83. doi: 10.1507/endocrj1954.33.375.
TSH receptor antibody (TRAb) activity using chicken thyroid receptor (c-TRAb) and porcine thyroid receptor (p-TRAb) was determined by the incubation of 125I-bovine TSH with each receptor. Both c-TRAb and p-TRAb activity in LATS positive and negative Graves' sera were compared. 15 out of 39 LATS positive sera and 4 out of 46 LATS negative sera had positive c-TRAb activity. On the other hand, all LATS positive sera and 33 out of 46 LATS negative sera had positive p-TRAb activity. No relationship between c-TRAb and p-TRAb activity was observed, and there was also no correlation between c-TRAb and LATS activity. Changes in c-TRAb, p-TRAb and LATS activity in the clinical course of patients with Graves' disease were examined. These activities were parallel in some cases, but in others they were not. A weak c-TRAb activity was observed in 4 out of 29 Hashimoto's disease, but all cases with thyroid cancer and subacute thyroiditis showed no activity. Sera with positive c-TRAb activity did not stimulate chicken thyroid in chick bioassay. These results suggest that some cases of TRAb in Graves' disease (mainly LATS) inhibit TSH binding to chicken thyroid receptor (non-mammalian species) in the same way as mammalian thyroid, but may not have any stimulatory action on thyroid hormone synthesis. It is interesting to note that TRAb including LATS have the similar effect on TSH receptor even in nonmammalian species.
通过将¹²⁵I-牛促甲状腺激素（TSH）与鸡甲状腺受体（c-TRAb）和猪甲状腺受体（p-TRAb）分别孵育，测定TSH受体抗体（TRAb）的活性。比较了格雷夫斯病患者LATS阳性和阴性血清中的c-TRAb和p-TRAb活性。39份LATS阳性血清中有15份、46份LATS阴性血清中有4份具有阳性c-TRAb活性。另一方面，所有LATS阳性血清和46份LATS阴性血清中的33份具有阳性p-TRAb活性。未观察到c-TRAb和p-TRAb活性之间的关系，c-TRAb与LATS活性之间也无相关性。研究了格雷夫斯病患者临床病程中c-TRAb、p-TRAb和LATS活性的变化。这些活性在某些情况下是平行的，但在其他情况下则不是。29例桥本氏病中有4例观察到较弱的c-TRAb活性，但所有甲状腺癌和亚急性甲状腺炎病例均无活性。c-TRAb活性阳性的血清在雏鸡生物测定中未刺激鸡甲状腺。这些结果表明，格雷夫斯病中某些TRAb病例（主要是LATS）以与哺乳动物甲状腺相同的方式抑制TSH与鸡甲状腺受体（非哺乳动物物种）的结合，但可能对甲状腺激素合成没有任何刺激作用。值得注意的是，包括LATS在内的TRAb即使在非哺乳动物物种中对TSH受体也有类似作用。
