Department of Neurology, Affiliated Hospital of Jining Medical University, Jining, Shandong 272000, PR China.
Department of Neurology, Affiliated Hospital of Jining Medical University, Jining, Shandong 272000, PR China.
J Neuroimmunol. 2018 Oct 15;323:136-142. doi: 10.1016/j.jneuroim.2018.06.018. Epub 2018 Jun 30.
To investigate if oxymatrine could ameliorate hippocampus ischemia/reperfusion (I/R) injury induced in rats and explore the possible mechanism. Rats were randomly divided into four groups: sham group, I/R group, I/R + OMT-treated group, I/R + Vehicle-treated group. Oxymatrine or vehicle solution was intraperitoneally injected OMT (150 mg/kg) 60 min before renal ischemia respectively. Water maze was measured; cell apoptosis was assessed by doing TUNEL assay and detecting the expression of P53, Bax, and Cleaved-Caspase-3; autophagy were assessed by measuring the expression of LC3 and P62. The expression of SIRT1 was also detected. Oxymatrine treatment alleviated histological injury in I/R rats, inhibiting apoptosis, promoting autophagy and accompanied by upregulated expression of SIRT1 proteins. Oxymatrine may attenuate hippocampus ischemia/reperfusion injury through upregulation SIRT1, further influencing the processes of apoptosis and autophagy.
探讨氧化苦参碱（OMT）是否能减轻大鼠海马缺血再灌注（I/R）损伤，并探讨其可能的机制。
将大鼠随机分为 4 组：假手术组、I/R 组、I/R+OMT 治疗组、I/R+载体治疗组。分别于肾缺血前 60min 腹腔注射 OMT（150mg/kg）或载体溶液。采用水迷宫法测定；TUNEL 法检测细胞凋亡，检测 P53、Bax 和 Cleaved-Caspase-3 的表达；通过检测 LC3 和 P62 的表达来评估自噬。还检测了 SIRT1 的表达。
氧化苦参碱治疗减轻了 I/R 大鼠的组织损伤，抑制了细胞凋亡，促进了自噬，并伴有 SIRT1 蛋白表达上调。
氧化苦参碱可能通过上调 SIRT1 减轻海马缺血再灌注损伤，进一步影响凋亡和自噬过程。
