Tamborska E, Insel T, Marangos P J
Eur J Pharmacol. 1986 Jul 31;126(3):281-7. doi: 10.1016/0014-2999(86)90058-0.
In this study the binding to 'central' and 'peripheral' benzodiazepine receptors in various brain areas in MR/N and MNR/N rat strains was investigated. The specific ligands [3H]BCCE and [3H]RO 5-4864 were used for binding to 'central' and 'peripheral' benzodiazepine receptors, respectively. Neither [3H]BCCE nor [3H]RO 5-4864 binding was changed in MNR/N strain in comparison to MR/N rat strain in cerebral cortex, cerebellum, hippocampus and medulla-pons. Scatchard analysis of [3H]BCCE or [3H]RO 5-4864 binding to cerebellar membranes also revealed no changes in either affinity or density in both types of benzodiazepine receptors. Autoradiographic analysis of brain regions using microdensitometry shows no differences in [3H]flunitrazepam binding between the MR/N and MNR/N strains. Our results, therefore, fail to confirm a previous report showing alterations in benzodiazepine receptors in Maudsley rats.
在本研究中，对MR/N和MNR/N大鼠品系不同脑区中“中枢”和“外周”苯二氮䓬受体的结合情况进行了研究。分别使用特异性配体[3H]BCCE和[3H]RO 5-4864与“中枢”和“外周”苯二氮䓬受体结合。与MR/N大鼠品系相比，在大脑皮质、小脑、海马和延髓脑桥中，MNR/N品系的[3H]BCCE和[3H]RO 5-4864结合均未发生变化。对小脑膜上[3H]BCCE或[3H]RO 5-4864结合进行的Scatchard分析也显示，两种类型的苯二氮䓬受体的亲和力或密度均未改变。使用显微密度测定法对脑区进行放射自显影分析表明，MR/N和MNR/N品系之间[3H]氟硝西泮的结合没有差异。因此，我们的结果未能证实先前一份显示莫兹利大鼠苯二氮䓬受体发生改变的报告。
