Seyedmajidi Maryam, Seifi Safoura, Moslemi Dariush, Mozaffari Seyyedeh-Fatemeh, Gholinia Hemmat, Zolfaghari Zahra
Dental Materials Research Center, Institue of Health, Babol University of Medical Sciences, Babol University of Medical Sciences, Babol, IR Iran.
Department of Oral and Maxillofacial Pathology, School of Dentistry, Babol University of Medical Sciences, Babol, IR Iran.
J Cancer Res Ther. 2018 Jul-Sep;14(5):964-969. doi: 10.4103/0973-1482.224350.
TWIST is a transcription factor that plays a key role in the development of primary tumor to metastatic stage of cancer. It is an inhibitor of E-cadherin in epithelial-to-mesenchymal transformation process (epithelial-mesenchymal transition). Few studies are available on the use of TWIST as a goal in molecular-targeted therapy. The aim of this study was to evaluate of TWIST expression in oral squamous cell carcinoma (OSCC) and its correlation with clinicopathologic factors.
In this cross-sectional study, immunohistochemical staining was for TWIST performed on 30 paraffin-embedded blocks of OSCC. Furthermore, thirty paraffin-embedded blocks of normal oral mucosa with minimum inflammation from the clinical and histopathologic aspects were selected. Staining intensity and percentage of stained cells from nuclear and cytoplasmic aspects were ranked in epithelial cells. TWIST expression and correlation with clinicopathologic factors were analyzed using Cox regression and Chi-square tests.
TWIST expression in OSCC was significantly increased compared to oral normal mucosa. Nuclear expression of TWIST in OSCC was significantly associated with clinical stage (P = 0.01) and lymph node metastasis (P = 0.007). Cytoplasmic expression of TWIST in OSCC was not associated with any clinicopathological factors.
The results support the role of TWIST in carcinogenesis, development of OSCC, and its metastasis to lymph nodes.
TWIST是一种转录因子，在癌症从原发性肿瘤发展到转移阶段的过程中起关键作用。它是上皮-间质转化过程（上皮-间质转变）中E-钙黏蛋白的抑制剂。关于将TWIST作为分子靶向治疗靶点的研究较少。本研究旨在评估TWIST在口腔鳞状细胞癌（OSCC）中的表达及其与临床病理因素的相关性。
在这项横断面研究中，对30个OSCC石蜡包埋组织块进行了TWIST免疫组化染色。此外，从临床和组织病理学角度选择了30个炎症最轻的正常口腔黏膜石蜡包埋组织块。对上皮细胞中染色强度以及细胞核和细胞质方面的染色细胞百分比进行分级。使用Cox回归和卡方检验分析TWIST表达及其与临床病理因素的相关性。
与正常口腔黏膜相比，OSCC中TWIST的表达显著增加。OSCC中TWIST的核表达与临床分期（P = 0.01）和淋巴结转移（P = 0.007）显著相关。OSCC中TWIST的细胞质表达与任何临床病理因素均无关联。
结果支持TWIST在OSCC的致癌作用、发展及其向淋巴结转移中的作用。
