Chauhan Ashutosh, Bhattacharyya Shalmoli, Ojha Rani, Mandal Arup K, Singh Shrawan K
Department of Urology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Department of Biophysics, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
J Cancer Res Ther. 2018 Jul-Sep;14(5):977-982. doi: 10.4103/jcrt.JCRT_942_15.
Renal cell carcinoma (RCC) is the most common kidney neoplasm and requires an early diagnosis because of poor response to conventional cancer treatments. However, till date, there is no reliable tumor marker available for the diagnosis of RCC.
The aim of the study was to evaluate the expression of speckle-type POZ protein (SPOP) as a biomarker in patients with RCC.
Blood samples were collected from fifty patients with RCC and ten healthy controls. Tumor tissue samples were obtained from nephrectomy specimen. Adjoining normal renal parenchyma of these fifty patients and eight normal renal tissue samples from normal kidney served as controls. Reverse transcriptase-polymerase chain reaction assay was performed for SPOP and mammalian target of rapamycin expression.
SPOP was significantly increased in blood of patients with RCC as compared to controls (0.754 ± 0.32 vs. 0.224 ± 0.14; P < 0.001). Twenty-two patients (44%) had SPOP value more than mean + 2 standard deviation (SD) of controls. In RCC tissue, 42 (84%) patients had increased expression of SPOP more than 0.523 (mean + 2 SD value of SPOP expression in controls). SPOP expression was high in blood of 60% patients and in tumor tissue of 90% patients with clear cell RCC. SPOP was higher in high grade and high stage of RCC.
Our result suggests that SPOP expression in blood might have a sensitivity that is low for routine diagnostic use and for screening for RCC. However, SPOP could be a potential tissue diagnostic biomarker in RCC.
肾细胞癌（RCC）是最常见的肾脏肿瘤，由于对传统癌症治疗反应不佳，需要早期诊断。然而，迄今为止，尚无可靠的肿瘤标志物可用于RCC的诊断。
本研究旨在评估斑点型POZ蛋白（SPOP）作为RCC患者生物标志物的表达情况。
收集50例RCC患者和10例健康对照者的血液样本。肿瘤组织样本取自肾切除标本。这50例患者的毗邻正常肾实质以及来自正常肾脏的8个正常肾组织样本作为对照。对SPOP和雷帕霉素哺乳动物靶标进行逆转录聚合酶链反应检测。
与对照组相比，RCC患者血液中的SPOP显著升高（0.754±0.32对0.224±0.14；P<0.001）。22例患者（44%）的SPOP值高于对照组平均值+2标准差（SD）。在RCC组织中，42例（84%）患者的SPOP表达增加超过0.523（对照组SPOP表达的平均值+2 SD值）。在透明细胞RCC患者中，60%的患者血液和90%的患者肿瘤组织中SPOP表达较高。RCC高级别和高分期患者的SPOP更高。
我们的结果表明，血液中SPOP的表达对于RCC的常规诊断和筛查可能具有较低的敏感性。然而，SPOP可能是RCC潜在的组织诊断生物标志物。
