Sajjadi Elham, Atashi Amir, Tajrishi Mona Agha Mohammad Hossein, Saei Zeinab
Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Stem Cell and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
J Cancer Res Ther. 2018 Jul-Sep;14(5):1079-1082. doi: 10.4103/0973-1482.187348.
Nowadays, noncoding RNAs are of special significance to scientists. Among RNAs, long noncoding RNAs (lncRNAs) have an important role in gene expression regulation. Recent studies show aberrant lncRNAs expression in different types of cancer including blood malignancies. As such, lncRNAs could be a possible way for diagnosis and treatment of certain cancers. In the current study, the level of PCA3 gene expression in patients with chronic myeloid leukemia (CML) was compared with normal individuals to find out whether the level of PCA3 in CML patients is increased according to our hypothesis.
The buffy coat was collected from peripheral blood of thirty untreated CML patients (BCR-ABL positive) and twenty normal volunteers. RNA was extracted from white blood cells and cDNA was synthesized. The level of PCA3 gene expression was measured using quantitative reverse transcription-polymerase chain reaction.
The results showed that PCA3 has expression in both normal and leukemic white blood cells. The data also revealed higher expression of PCA3 in leukemic patients, compared to the normal counterpart (P < 0.05).
The unusual increase in PCA3 gene expression in CML patients suggests the need for more research on mechanisms of molecular pathways related to PCA3 which could help achieve better treatment.
如今，非编码RNA对科学家具有特殊意义。在RNA中，长链非编码RNA（lncRNA）在基因表达调控中发挥着重要作用。最近的研究表明，lncRNA在包括血液恶性肿瘤在内的不同类型癌症中表达异常。因此，lncRNA可能是某些癌症诊断和治疗的一种途径。在本研究中，将慢性髓性白血病（CML）患者的PCA3基因表达水平与正常个体进行比较，以根据我们的假设确定CML患者中PCA3水平是否升高。
从30例未经治疗的CML患者（BCR-ABL阳性）和20名正常志愿者的外周血中采集血沉棕黄层。从白细胞中提取RNA并合成cDNA。使用定量逆转录-聚合酶链反应测量PCA3基因表达水平。
结果表明，PCA3在正常和白血病白细胞中均有表达。数据还显示，与正常对照组相比，白血病患者中PCA3的表达更高（P < 0.05）。
CML患者中PCA3基因表达的异常增加表明需要对与PCA3相关的分子途径机制进行更多研究，这有助于实现更好的治疗。
