精浆无助于酚红在母马子宫输卵管结合部的转运。
Seminal plasma does not aid in the transport of phenolsulfonphthalein across the uterotubal junction in mares.
作者信息
Ross Kayla A, Kolb David S, Macedo Alysson, Anderson Marion, Klein Claudia
机构信息
University of Calgary, Faculty of Veterinary Medicine, 3280 Hospital Drive NW, Calgary, Alberta T2N 4Z6 (Ross, Macedo, Klein); Lodi Veterinary Care, 705 N Main Street, Lodi Wisconsin 53555, USA (Kolb); Olds College, 4500-50th Street, Olds, Alberta T4H 1R6 (Anderson).
出版信息
Can Vet J. 2018 Sep;59(9):988-992.
This study tested the hypothesis that the presence of prostaglandin E2 in seminal plasma would aid in the transport of phenolsulfonphthalein (PSP) across the uterotubal junction. Five mares in estrus were inseminated during estrus with PSP dissolved in phosphate-buffered saline and during the subsequent estrus with PSP added to a standard insemination dose. Serum and urine samples were obtained at hours 0, 1, 2, and 3 following treatment and examined for the presence of PSP. Phenolsulfonphthalein could not be detected in any of the urine samples collected from mares following either treatment. None of the serum samples collected following intrauterine installation of PSP in PBS contained PSP. Phenolsulfonphthalein was detected in serum samples from 1 mare following insemination with semen containing PSP. Components in seminal plasma such as PGE2 did not facilitate the transport of PSP across the uterotubal junction as had been hypothesized.
本研究检验了以下假设
精浆中前列腺素E2的存在有助于酚红（PSP）穿过子宫输卵管连接部。5匹处于发情期的母马在发情期用溶解于磷酸盐缓冲液的PSP进行输精，并在随后的发情期将PSP添加到标准输精剂量中。在处理后的0、1、2和3小时采集血清和尿液样本，并检测其中PSP的存在情况。两种处理后从母马采集的任何尿液样本中均未检测到酚红。在子宫内注入PSP于磷酸盐缓冲液后的血清样本中均未检测到PSP。在1匹母马用含PSP的精液输精后的血清样本中检测到了酚红。精浆中的成分如前列腺素E2并未如假设的那样促进PSP穿过子宫输卵管连接部。
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