Lv Ke, Chen Zhenyu, Zhang Xiaoqing, Zhang Quanbin, Liu Ling
Neurosurgical Department, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200092, P.R. China.
Neuroregeneration Key Laboratory of Shanghai Universities, Tongji University School of Medicine, Shanghai 200092, P.R. China.
Oncol Lett. 2018 Oct;16(4):4567-4576. doi: 10.3892/ol.2018.9154. Epub 2018 Jul 17.
Most of the brain tumors are malignant with an extremely poor survival rate. Recent progress in identifying cancer stem cells (CSCs) within the brain tumors is starting to revolutionize our understanding in the imitation and progression of tumors as well as relapse and the development of therapeutic strategies. Suspension spheroid body culture paradigm is a routine method in enriching CSCs. While, it was reported recently that CSCs within the brain tumor may also be enriched through adherent monolayer culture with optimized properties. In the present study, 18 surgically resected brain tumors were used for analyzing the feasibility of adherent enrichment of CSCs. The results indicated that 50% of glioblastomas were able to generate adherent CSCs, which were uniformly positive for Sox2, CD133, GFAP and Nestin. However, adherent culture paradigm failed to yield CSCs in secondary brain tumors, including neurocytomas, ependymomas, germ cell tumors or low-grade astrocytomas, which is most likely due to a lack of CD133/Sox2 cells within the original biopsies. Therefore, it was concluded that the adherent culture paradigm may serve as a reliable method in enriching brain CSCs, but this method is more suitable for enriching CD133/Sox2 CSCs in glioblastomas.
大多数脑肿瘤是恶性的，生存率极低。最近在脑肿瘤中识别癌症干细胞（CSCs）方面取得的进展，正开始彻底改变我们对肿瘤的发生、发展以及复发和治疗策略制定的理解。悬浮球体培养模式是富集CSCs的常规方法。然而，最近有报道称，脑肿瘤中的CSCs也可以通过具有优化特性的贴壁单层培养来富集。在本研究中，使用18例手术切除的脑肿瘤来分析贴壁富集CSCs的可行性。结果表明，50%的胶质母细胞瘤能够产生贴壁CSCs，这些细胞对Sox2、CD133、GFAP和巢蛋白均呈阳性。然而，贴壁培养模式未能在继发性脑肿瘤中产生CSCs，包括神经细胞瘤、室管膜瘤、生殖细胞瘤或低级别星形细胞瘤，这很可能是由于原始活检组织中缺乏CD133/Sox2细胞。因此，得出的结论是，贴壁培养模式可能是富集脑CSCs的可靠方法，但这种方法更适合于富集胶质母细胞瘤中的CD133/Sox2 CSCs。
