Qian Wei-bin, Zhang Li, Wang Sheng-rui, Cao Chang-chun, Li Yan-ping, Cheng Jie, Yang Jia-chun, Li Wen-zhang
Guang Pu Xue Yu Guang Pu Fen Xi. 2016 Nov;36(11):3608-14.
UV-Vis absorbance, fluorescence, and Gas Chromatography Mass Spectrometry (GC-MS) were applied to the comparative study on sediment dissolved organic nitrogen (DON) in five typical lakes (Erhai lake, Dianchilake, Poyang lake, Wuhan Dong lake, and Taihu lake) in different lake regions with different nutrition status, revealing the relationship between structural and compositional characteristics of sediment DON and trophic level of lakes. The obtained results showed that: ①Structure of lake sediment DON in Yungui Plateau region is more stable, compared with those in Eastern Plain region, indicating its lower bioavailability. ②In Yungui Plateau region, the source and compositional characteristics of sediment DON weremore complex in Dianchi lake (a seriously polluted lake), and its sediment DON bioavailabilitywas relatively higher. While, with respect to the less polluted Erhai lake, the source of sediment DON is more simple with a higher stability in DON structure and composition, which is beneficial for maintaining its good water quality. ③In Eastern Plain region, nutrition status of Taihu lake was similer to Donghu lake. The structure and composition of sediment DON was complex. But the lower aromaticityand fewer Aromatic ring substituents abundance made their relatively weak nutrient retention ability, posing risk to water quality. With regard to Poyang Lake, the structure and composition of sediment DON was relatively simple, but nutrient retention ability was relatively strong, which played a positive role in maintaining good water quality. ④P(Ⅲ+Ⅴ, n)/P(Ⅰ+Ⅱ, n) values(the content ratio of humic-like substanceto protein-like substances)were in sequence of Dianchi Lake (33.14)>Erhai Lake(21.49)>Taihu Lake(9.06)>Donghu Lake(7.04)>Poyang Lake(4.83), while E(4)/E(6) values (the ratio of UV-Vis absorbance at 465 and 665 nm) were in sequence of Dianchi Lake (27.00)>Donghu Lake(6.65)>Poyang Lake(5.47)>Taihu(3.50)>Erhai Lake(2.31). In addition, P(Ⅲ+Ⅴ, n)/P(Ⅰ+Ⅱ, n) and E(4)/E(6) valueswere positively correlated with thecontents of the different nitrogen (N) forms in the sediments. The above information suggested that P(Ⅲ+Ⅴ, n)/P(Ⅰ+Ⅱ, n) and E(4)/E(6) values exhibited good discrimination degree among different trophic status lakes, and they were considered to indirectly indicate the nutrition levels of lakes to a certain extent.
采用紫外可见分光光度法、荧光分析法和气相色谱 - 质谱联用法（GC - MS），对不同湖区、不同营养状态的五个典型湖泊（洱海、滇池、鄱阳湖、武汉东湖和太湖）沉积物中的溶解有机氮（DON）进行了对比研究，揭示了沉积物DON的结构和组成特征与湖泊营养水平之间的关系。研究结果表明：①与东部平原地区湖泊相比，云贵高原地区湖泊沉积物DON的结构更稳定，表明其生物可利用性较低。②在云贵高原地区，滇池（一个污染严重的湖泊）沉积物DON的来源和组成特征更为复杂，其沉积物DON的生物可利用性相对较高。而对于污染较轻的洱海，沉积物DON的来源较为单一，DON结构和组成的稳定性较高，这有利于维持其良好的水质。③在东部平原地区，太湖和东湖的营养状态相似。沉积物DON的结构和组成较为复杂。但其较低的芳香性和较少的芳环取代基丰度使其营养保留能力相对较弱，对水质构成风险。对于鄱阳湖，沉积物DON的结构和组成相对简单，但其营养保留能力相对较强，这对维持良好水质起到了积极作用。④P(Ⅲ + Ⅴ, n)/P(Ⅰ + Ⅱ, n)值（类腐殖质与类蛋白质物质的含量比）依次为滇池（33.14）>洱海（21.49）>太湖（9.06）>东湖（7.04）>鄱阳湖（4.83），而E(4)/E(6)值（465和665nm处紫外可见吸光度之比）依次为滇池（27.00）>东湖（6.65）>鄱阳湖（5.47）>太湖（3.50）>洱海（2.31）。此外，P(Ⅲ + Ⅴ, n)/P(Ⅰ + Ⅱ, n)和E(4)/E(6)值与沉积物中不同形态氮（N）的含量呈正相关。上述信息表明，P(Ⅲ + Ⅴ, n)/P(Ⅰ + Ⅱ, n)和E(4)/E(6)值在不同营养状态湖泊之间具有良好的区分度，在一定程度上可间接指示湖泊的营养水平。
