99mTc-锡-磷酸盐聚乙烯吡咯烷酮稳定胶体的制备及其在骨髓中的分布。

Preparation of 99mTc-tin-phosphate polyvinyl pyrollidone stabilized colloid and distribution in bone marrow.

作者信息

Shahid M A, Imran S L, Ashraf M, Akhtar M W

出版信息

Int J Rad Appl Instrum A. 1986;37(3):249-55. doi: 10.1016/0883-2889(86)90181-4.

PMID:3019937

Abstract

Technetium-99m-Sn-phosphate colloid was prepared in the presence of polyvinylpyrollidone(PVP), molecular weight 44,000, for bone marrow imaging. Size of the colloidal particles, as determined by coulter counter, microphotographic and electron microscopic studies was 15-35 nm. The colloid preparation was checked for the presence of any soluble components by Sephadex G-25 chromatography. Scintigraphy in rabbits showed a high concentration of the colloid in the bone marrow. Tissue distribution studies in rabbits showed 26.7% of the injected dose at 1 h post-injection in the bone marrow collected from femoral shaft and head. Although liver and spleen showed considerable levels of activity, kidneys and compact bone did not show any uptake. The colloid cleared from the blood exponentionally with a first phase showing a relatively fast clearance while in the second phase it disappeared more slowly. Uptake of the colloid in human bone marrow was close to that in the rabbit and thus clinical evaluation is warranted.

摘要

在分子量为44000的聚乙烯吡咯烷酮（PVP）存在的情况下制备了用于骨髓显像的锝-99m-锡-磷酸盐胶体。通过库尔特计数器、显微照相和电子显微镜研究确定的胶体颗粒大小为15 - 35纳米。通过葡聚糖凝胶G - 25色谱法检查胶体制剂中是否存在任何可溶性成分。兔的闪烁显像显示骨髓中胶体浓度很高。兔的组织分布研究显示，在注射后1小时，从股骨干和股骨头采集的骨髓中注射剂量的26.7%存在于其中。尽管肝脏和脾脏显示出相当高的活性水平，但肾脏和密质骨未显示任何摄取。胶体从血液中呈指数清除，第一阶段显示相对快速的清除，而在第二阶段清除较慢。该胶体在人骨髓中的摄取与兔相近，因此有必要进行临床评估。