School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China.
School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China.
Colloids Surf B Biointerfaces. 2018 Dec 1;172:480-486. doi: 10.1016/j.colsurfb.2018.08.070. Epub 2018 Sep 1.
In this work, the effect of silica nanoparticles (NPs) adding to DPPC monolayer and the interaction between DPPC and silica nanoparticles are studied. Silica nanoparticles are prepared by microemulsion, meanwhile, DMDCS and APTES are used to modify silica NPs to get three types of modified silica NPs. These samples are mixed with DPPC to form mixed monolayer. By using the atomic force microscope (AFM), surface pressure-area and pressure-time isotherms, the effects of different hydrophilic-hydrophobic silica nanoparticles on the interface of lipid monolayer is analyzed. The data shows that the addition of silica nanoparticles changes the phase behavior, the collapse time and the structure of monolayer. Hydrophilic silica NPs decreases the collapse pressure and rigidity of DPPC monolayer, and makes monolayer collapse earlier since the steric hindrance leads to the resistance to compression, while hydrophobic silica NPs have less effect on monolayer in collapse pressure or rigidity but the texture of monolayer, and the addition of hydrophobic NPs causes the appearance of holes in the monolayer. We suppose that there are several possible locations of hydrophobic and hydrophilic silica nanoparticles in the air-water interface, which leads to different effects on the structure and rheological behavior of monolayer. This study can deepen the understanding on how nanoparticles affect human body since industries of nanoparticles on drug delivery, oil recovery and floatation are developing rapidly and getting more and more outside interest on a daily basis.
在这项工作中，研究了二氧化硅纳米粒子（NPs）对 DPPC 单层的影响以及 DPPC 和二氧化硅纳米粒子之间的相互作用。通过微乳液法制备了二氧化硅纳米粒子，同时，使用 DMDCS 和 APTES 对二氧化硅 NPs 进行改性，得到了三种类型的改性二氧化硅 NPs。将这些样品与 DPPC 混合形成混合单层。通过原子力显微镜（AFM），表面压力-面积和压力-时间等温线，分析了不同亲水性-疏水性二氧化硅纳米粒子对脂质单层界面的影响。数据表明，纳米粒子的加入改变了相行为、单层的坍塌时间和结构。亲水性二氧化硅 NPs 降低了 DPPC 单层的坍塌压力和刚性，使其更早坍塌，因为位阻导致压缩阻力，而疏水性二氧化硅 NPs 对单层的坍塌压力或刚性影响较小，但对单层的结构有影响，并且疏水性 NPs 的加入会导致单层出现孔。我们假设疏水性和亲水性二氧化硅纳米粒子在气-水界面上可能有几个位置，这导致了对单层结构和流变行为的不同影响。由于纳米粒子在药物输送、采油和浮选等领域的工业正在迅速发展，并且每天都有越来越多的人对其产生兴趣，因此，这项研究可以加深对纳米粒子如何影响人体的理解。
