恶臭假单胞菌RE204中一个携带指定异丙苯代谢基因的20千碱基DNA片段的自发缺失。
Spontaneous deletion of a 20-kilobase DNA segment carrying genes specifying isopropylbenzene metabolism in Pseudomonas putida RE204.
作者信息
Eaton R W, Timmis K N
出版信息
J Bacteriol. 1986 Oct;168(1):428-30. doi: 10.1128/jb.168.1.428-430.1986.
Abstract
The genes encoding isopropylbenzene metabolism in Pseudomonas putida RE204 are readily lost in two ways: by loss (curing) of plasmid pRE4 which specifies the catabolic pathway and by deletion from pRE4 of an approximately 20-kilobase segment of DNA carrying the catabolic genes. The presence of DNA sequences at the ends of the catabolic gene region sharing homology with one another suggests that the deletions result from recombination events between these homologous sequences.
摘要
恶臭假单胞菌RE204中编码异丙苯代谢的基因很容易通过两种方式丢失：一种是通过丢失（消除）指定分解代谢途径的质粒pRE4，另一种是从携带分解代谢基因的约20千碱基DNA片段中从pRE4上删除。分解代谢基因区域末端相互具有同源性的DNA序列的存在表明，这些缺失是由这些同源序列之间的重组事件导致的。
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