Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Metallurgical Engineering, Universitat Politècnica de Catalunya (UPC), 08019 Barcelona, Spain.
Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya (UPC), 08019 Barcelona, Spain.
Int J Mol Sci. 2018 Aug 30;19(9):2574. doi: 10.3390/ijms19092574.
In this study, highly-interconnected porous titanium implants were produced by powder sintering with different porous diameters and open interconnectivity. The actual foams were produced using high cost technologies: Chemical Vapor Deposition (CVD), Physical Vapor Deposition (PVD), and spark plasma sintering, and the porosity and/or interconnection was not optimized. The aim was to generate a bioactive surface on foams using two different strategies, based on inorganic thermo-chemical treatment and organic coating by peptide adsorption, to enhance osseointegration. Porosity was produced using NaCl as a space holder and polyethyleneglicol as a binder phase. Static and fatigue tests were performed in order to determine mechanical behaviors. Surface bioactivation was performed using a thermo-chemical treatment or by chemical adsorption with peptides. Osteoblast-like cells were cultured and cytotoxicity was measured. Bioactivated scaffolds and a control were implanted in the tibiae of rabbits. Histomorphometric evaluation was performed at 4 weeks after implantation. Interconnected porosity was 53% with an average diameter of 210 µm and an elastic modulus of around 1 GPa with good mechanical properties. The samples presented cell survival values close to 100% of viability. Newly formed bone was observed inside macropores, through interconnected porosity, and on the implant surface. Successful bone colonization of inner structure (40%) suggested good osteoconductive capability of the implant. Bioactivated foams showed better results than non-treated ones, suggesting both bioactivation strategies induce osteointegration capability.
在这项研究中，通过粉末烧结制备了具有不同孔径和连通性的高度互联多孔钛植入物。实际的泡沫体是使用高成本技术生产的：化学气相沉积（CVD）、物理气相沉积（PVD）和火花等离子体烧结，并且孔隙率和/或连通性没有得到优化。目的是使用两种不同的策略在泡沫体上生成生物活性表面，一种是基于无机热化学处理的策略，另一种是基于肽吸附的有机涂层策略，以增强骨整合。使用 NaCl 作为空间保持剂和聚乙二醇作为结合相来产生多孔性。进行了静态和疲劳测试，以确定机械性能。通过热化学处理或通过肽化学吸附进行表面生物活化。培养成骨细胞样细胞并测量细胞毒性。将生物活化支架和对照物植入兔胫骨中。植入后 4 周进行组织形态计量学评估。连通孔隙率为 53%，平均孔径为 210 µm，弹性模量约为 1 GPa，具有良好的机械性能。样品的细胞存活率接近 100%。在大孔内、通过连通孔隙和在植入物表面观察到新形成的骨。内部结构（40%）的成功骨定植表明植入物具有良好的骨传导能力。生物活化泡沫体的结果优于未经处理的泡沫体，这两种生物活化策略都能诱导骨整合能力。
