Guillem-Marti Jordi, Vidal Elia, Girotti Alessandra, Heras-Parets Aina, Torres Diego, Arias Francisco Javier, Ginebra Maria-Pau, Rodriguez-Cabello Jose Carlos, Manero Jose Maria
Biomaterials, Biomechanics and Tissue Engineering (BBT), Department of Materials Science and Engineering (CEM), Universitat Politècnica de Catalunya-BarcelonaTech (UPC), 08019 Barcelona, Spain.
Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya-BarcelonaTech (UPC), 08019 Barcelona, Spain.
Pharmaceutics. 2023 Mar 8;15(3):872. doi: 10.3390/pharmaceutics15030872.
The 3D printing of titanium (Ti) offers countless possibilities for the development of personalized implants with suitable mechanical properties for different medical applications. However, the poor bioactivity of Ti is still a challenge that needs to be addressed to promote scaffold osseointegration. The aim of the present study was to functionalize Ti scaffolds with genetically modified elastin-like recombinamers (ELRs), synthetic polymeric proteins containing the elastin epitopes responsible for their mechanical properties and for promoting mesenchymal stem cell (MSC) recruitment, proliferation, and differentiation to ultimately increase scaffold osseointegration. To this end, ELRs containing specific cell-adhesive (RGD) and/or osteoinductive (SN15) moieties were covalently attached to Ti scaffolds. Cell adhesion, proliferation, and colonization were enhanced on those scaffolds functionalized with RGD-ELR, while differentiation was promoted on those with SN15-ELR. The combination of both RGD and SN15 into the same ELR stimulated cell adhesion, proliferation, and differentiation, although at lower levels than those for every single moiety. These results suggest that biofunctionalization with SN15-ELRs could modulate the cellular response to improve the osseointegration of Ti implants. Further investigation on the amount and distribution of RGD and SN15 moieties in ELRs could improve cell adhesion, proliferation, and differentiation compared to the present study.
钛（Ti）的3D打印为开发具有适合不同医学应用机械性能的个性化植入物提供了无数可能性。然而，Ti的生物活性较差仍然是一个需要解决的挑战，以促进支架骨整合。本研究的目的是用基因工程改造的类弹性蛋白重组体（ELR）对Ti支架进行功能化，ELR是一种合成聚合物蛋白，含有赋予其机械性能并促进间充质干细胞（MSC）募集、增殖和分化的弹性蛋白表位，最终增强支架骨整合。为此，将含有特定细胞黏附（RGD）和/或骨诱导（SN15）部分的ELR共价连接到Ti支架上。在用RGD-ELR功能化的支架上，细胞黏附、增殖和定植得到增强，而在用SN15-ELR功能化的支架上，细胞分化得到促进。将RGD和SN15组合在同一ELR中可刺激细胞黏附、增殖和分化，尽管其水平低于每个单一部分。这些结果表明，用SN15-ELR进行生物功能化可调节细胞反应，以改善Ti植入物的骨整合。与本研究相比，进一步研究ELR中RGD和SN15部分的数量和分布可能会改善细胞黏附、增殖和分化。
