Elmonem Mohamed A, Berlingerio Sante Princiero, van den Heuvel Lambertus P, de Witte Peter A, Lowe Martin, Levtchenko Elena N
Department of Pediatric Nephrology & Development and Regeneration, University Hospitals Leuven, KU Leuven-University of Leuven, Herestraat 49, Box 817, 3000 Leuven, Belgium.
Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, 11628 Cairo, Egypt.
Cells. 2018 Sep 1;7(9):130. doi: 10.3390/cells7090130.
The structural and functional similarity of the larval zebrafish pronephros to the human nephron, together with the recent development of easier and more precise techniques to manipulate the zebrafish genome have motivated many researchers to model human renal diseases in the zebrafish. Over the last few years, great advances have been made, not only in the modeling techniques of genetic diseases in the zebrafish, but also in how to validate and exploit these models, crossing the bridge towards more informative explanations of disease pathophysiology and better designed therapeutic interventions in a cost-effective in vivo system. Here, we review the significant progress in these areas giving special attention to the renal phenotype evaluation techniques. We further discuss the future applications of such models, particularly their role in revealing new genetic diseases of the kidney and their potential use in personalized medicine.
斑马鱼幼体前肾与人类肾单位在结构和功能上的相似性，以及近年来操控斑马鱼基因组的技术变得更加简便和精确，促使许多研究人员利用斑马鱼建立人类肾脏疾病模型。在过去几年中，不仅在斑马鱼遗传疾病的建模技术方面取得了巨大进展，而且在如何验证和利用这些模型方面也取得了很大进展，从而跨越了一道桥梁，迈向在具有成本效益的体内系统中对疾病病理生理学进行更丰富的解释以及设计出更优的治疗干预措施。在此，我们回顾这些领域的重大进展，特别关注肾脏表型评估技术。我们还将进一步讨论此类模型的未来应用，尤其是它们在揭示新的肾脏遗传疾病方面的作用以及在个性化医疗中的潜在用途。
