Jiang Xiao-Hui, Chen Li-Na, Li Kai, Guo Yuan, Zheng Zhong-Yuan, Yang Ting, Zheng Xiao-Jun, Li Yu-Jie, Zhu Xiao-Xin
Anhui University of Chinese Medicine， Hefei 230038， China.
Research Center of Artemisinin, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2018 Aug;43(15):3051-3057. doi: 10.19540/j.cnki.cjcmm.20180608.002.
Cerebral malaria (CM) is the leading cause of death in children under 5 years in Africa, severe neurological sequelae may occur in surviving children. Although artesunate has made breakthrough progress in the clinical treatment of CM, the clinical problems of high mortality and high morbidity have not yet been completely resolved. In this study, an experimental cerebral malaria (ECM) model was established by infecting C57BL/6 mice with Pb ANKA (Plasmodium berghei ANKA) to compare parasitemia level, survival rates, and rapid murine coma behavior scale scores, cerebral microvascular obstruction, haemozoin deposition in the liver, body temperature and weight to investigate the anti-cerebral malaria effect of the artesunate compound combination. The results showed that the artesunate compound combination could improve the survival rate of Pb ANKA-infected mice, reduce the level of parasitemia, effectively improve the symptoms of ECM neurological injury, reduce cerebrovascular obstruction and haemozoin deposition in the liver, and also significantly improve body temperature, weight and other basic indicators. The results showed that the artesunate compound combination improved the pathological changes and neurological damage caused by CM. It is expected to provide a theoretical basis for human cerebral malaria patients in clinical adjuvant therapy.
脑型疟疾（CM）是非洲5岁以下儿童死亡的主要原因，幸存儿童可能会出现严重的神经后遗症。尽管青蒿琥酯在CM的临床治疗上取得了突破性进展，但高死亡率和高发病率的临床问题尚未得到彻底解决。在本研究中，通过用伯氏疟原虫ANKA（Pb ANKA）感染C57BL/6小鼠建立实验性脑型疟疾（ECM）模型，比较疟原虫血症水平、生存率、快速小鼠昏迷行为量表评分、脑微血管阻塞、肝脏中疟色素沉积、体温和体重，以研究青蒿琥酯复方组合的抗脑型疟疾作用。结果表明，青蒿琥酯复方组合可提高Pb ANKA感染小鼠的生存率，降低疟原虫血症水平，有效改善ECM神经损伤症状，减少脑血管阻塞和肝脏中疟色素沉积，还能显著改善体温、体重等基本指标。结果表明，青蒿琥酯复方组合改善了CM引起的病理变化和神经损伤。有望为人类脑型疟疾患者临床辅助治疗提供理论依据。
