基于GPER2/MAPK/STAT1轴探讨少腹逐瘀汤治疗子宫内膜异位症痛经的分子机制
[Molecular mechanism of Shaofu Zhuyu decoction in treating endometriosis dysmenorrhea based on GPER2/MAPK/STAT1 axis].
作者信息
Cui Yu-Hong, Wu Jing, Cai Wei, Du Juan, Cao Jun-Min, Ji Xiu-Jia, Gong Zi-Han
机构信息
Gansu University of Chinese Medicine, Lanzhou 730000， China.
Gansu Provincial Hospital, Lanzhou 730000, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2018 Aug;43(16):3362-3367. doi: 10.19540/j.cnki.cjcmm.20180408.004.
The aim of this paper is to study the molecular mechanism of Shaofu Zhuyu decoction in treating dysmenorrhea of endometriosis based on GPER2/MAPK/STAT1 axis. In this study, HE staining was used to observe the pathological changes of the rats in each group. The levels of TNF-α and IL-6 were detected by ELISA assay. The mRNA expressions of neurotransmitter receptor (NK1) and GPER were detected by qPCR. The protein contents of MAPK and STAT1 were detected by Western blot. According to the results, compared with the model group, Shaofu Zhuyu decoction could significantly improve the inflammation of the ectopic uterine cavity tissue, decrease the contents of TNF-α and IL-6 in the uterine cavity, the mRNA expressions of NK1 and GPER, and the protein expressions of MAPK and STAT1. In conclusion, Shaofu Zhuyu decoction could effectively inhibit the expressions of GPER2, MAPK and STAT1, decrease the levels of TNF-α, IL-6 and NK1 mRNA and relieve the inflammatory pain in patients with endometriosis.
本文旨在基于GPER2/MAPK/STAT1轴研究少腹逐瘀汤治疗子宫内膜异位症痛经的分子机制。在本研究中，采用苏木精-伊红（HE）染色观察各组大鼠的病理变化。通过酶联免疫吸附测定（ELISA）法检测肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的水平。通过定量聚合酶链反应（qPCR）检测神经递质受体（NK1）和G蛋白偶联雌激素受体（GPER）的mRNA表达。通过蛋白质免疫印迹法（Western blot）检测丝裂原活化蛋白激酶（MAPK）和信号转导与转录激活因子1（STAT1）的蛋白质含量。结果显示，与模型组相比，少腹逐瘀汤可显著改善异位子宫腔组织的炎症，降低子宫腔内TNF-α和IL-6的含量、NK1和GPER的mRNA表达以及MAPK和STAT1的蛋白质表达。综上所述，少腹逐瘀汤可有效抑制GPER2、MAPK和STAT1的表达，降低TNF-α、IL-6水平及NK1 mRNA水平，缓解子宫内膜异位症患者的炎性疼痛。
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