Laboratorio de Neurociencias Moleculares e Integrativas, Escuela de Medicina, División Ciencias de la Salud, Universidad Anáhuac Mayab, Mérida, Yucatán, Mexico; Intercontinental Neuroscience Research Group.
Depto. de Neuropatología Molecular, División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, México, Mexico.
Brain Res. 2018 Dec 1;1700:181-189. doi: 10.1016/j.brainres.2018.09.006. Epub 2018 Sep 7.
Obesity is the result of the interaction of multiple variables, including the excessive increase of sugar-sweetened beverages consumption. Diets aimed to treat obesity have suggested the use of artificial sweeteners. However, recent evidence has shown several health deficits after intake of artificial sweeteners, including effects in neuronal activity. Therefore, the influence of artificial sweeteners consumption such as Splenda, on the expression of c-Fos and neuronal nuclear protein (NeuN) in hypothalamus and hippocampus remains to be determined.
We investigated the effects on c-Fos or NeuN expression in hypothalamus and hippocampus of Splenda-treated rats.
Splenda was diluted in water (25, 75 or 250 mg/100 mL) and orally given to rats during 2 weeks ad libitum. Next, animals were sacrificed by decapitation and brains were collected for analysis of c-Fos or NeuN immunoreactivity.
Consumption of Splenda provoked an inverted U-shaped dose-effect in c-Fos expression in ventromedial hypothalamic nucleus while similar findings were observed in dentate gyrus of hippocampus. In addition, NeuN immunoreactivity was enhanced in ventromedial hypothalamic nucleus at 25 or 75 mg/100 mL of Splenda intake whereas an opposite effect was observed at 250 mg/100 mL of artificial sweetener consumption. Lastly, NeuN positive neurons were increased in CA2/CA3 fields of hippocampus from Splenda-treated rats (25, 75 or 250 mg/100 mL).
Consuming Splenda induced effects in neuronal biomarkers expression. To our knowledge, this study is the first description of the impact of intake Splenda on c-Fos and NeuN immunoreactivity in hypothalamus and hippocampus in rats.
肥胖是多种变量相互作用的结果，包括糖饮料消费的过度增加。旨在治疗肥胖的饮食建议使用人工甜味剂。然而，最近的证据表明，摄入人工甜味剂后会对健康产生多种负面影响，包括对神经元活动的影响。因此，人工甜味剂（如 Splenda）的摄入对下丘脑和海马神经元 c-Fos 和核蛋白（NeuN）表达的影响仍有待确定。
我们研究了 Splenda 对大鼠下丘脑和海马中 c-Fos 或 NeuN 表达的影响。
将 Splenda 稀释在水中（25、75 或 250mg/100mL），并在 2 周内自由给予大鼠口服。然后，通过断头术处死动物，收集大脑用于分析 c-Fos 或 NeuN 免疫反应性。
Splenda 的摄入引起了腹内侧下丘脑核中 c-Fos 表达的倒 U 型剂量效应，而在海马齿状回也观察到类似的发现。此外，在摄入 25 或 75mg/100mL Splenda 时，下丘脑腹内侧核中的 NeuN 免疫反应性增强，而在摄入 250mg/100mL 人工甜味剂时则观察到相反的效果。最后，在摄入 Splenda（25、75 或 250mg/100mL）的大鼠海马 CA2/CA3 区中，NeuN 阳性神经元增加。
摄入 Splenda 会引起神经元生物标志物表达的变化。据我们所知，本研究首次描述了 Splenda 对大鼠下丘脑和海马中 c-Fos 和 NeuN 免疫反应性的影响。
