Wang Liqing, Yu Chao, Sun Xiaobo, Chan Sun-On
Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
Center of Health Examination, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.
Neurosci Lett. 2018 Nov 1;686:106-111. doi: 10.1016/j.neulet.2018.09.012. Epub 2018 Sep 7.
Our previous studies showed interaction of Nogo at the midline with its receptor (NgR) on optic axons plays a role in axon divergence at the mouse optic chiasm. Since NgR lacks a cytoplasmic domain, it needs transmembrane receptor partners for signal transduction. In this study, we examined whether the co-receptors of NgR, low-affinity neurotrophic receptor (p75) and Lingo-1, are localized on axons in the mouse optic pathway. In the retina, p75 and Lingo-1 were observed on neuroepithelial cells at E13 and later on the retinal ganglion cells at E14 and E15. At the optic disc, p75 was observed on the retinal axons, whereas Lingo-1 was found on glial processes surrounding the axon fascicles. Both p75 and Lingo-1 were found on axons in the optic stalk, optic chiasm and optic tract. Furthermore, a transient expression of Lingo-1 was observed on the SSEA-1 positive chiasmatic neurons at E13, but not at later developmental stages. The presence of p75 and Lingo-1 on optic axons provides further supports to the contribution of Nogo/NgR signaling in axon divergence at the mouse optic chiasm.
我们之前的研究表明，中线处的Nogo与其在视神经轴突上的受体（NgR）之间的相互作用在小鼠视交叉处的轴突发散中起作用。由于NgR缺乏细胞质结构域，它需要跨膜受体伙伴进行信号转导。在本研究中，我们检测了NgR的共受体——低亲和力神经营养受体（p75）和Lingo-1是否定位于小鼠视觉通路的轴突上。在视网膜中，E13时在神经上皮细胞上观察到p75和Lingo-1，随后在E14和E15时在视网膜神经节细胞上观察到。在视盘处，在视网膜轴突上观察到p75，而在轴突束周围的神经胶质突起上发现Lingo-1。在视柄、视交叉和视束的轴突上均发现了p75和Lingo-1。此外，在E13时，在SSEA-1阳性的交叉神经元上观察到Lingo-1的短暂表达，但在随后的发育阶段未观察到。视神经轴突上p75和Lingo-1的存在为Nogo/NgR信号在小鼠视交叉处轴突发散中的作用提供了进一步的支持。
