Kulkarni Ashwini N, Bhalkar Bhumika N, Khandare Rahul V, Kurade Mayur B, Jeon Byong-Hun, Govindwar Sanjay P
Department of Microbiology, Shivaji University, Kolhapur 416004, Maharashtra, India.
Department of Biotechnology, Shivaji University, Kolhapur 416004, Maharashtra, India.
J Biosci Bioeng. 2019 Mar;127(3):388-393. doi: 10.1016/j.jbiosc.2018.08.001. Epub 2018 Sep 7.
Densitometric high performance thin layer chromatography (HPTLC) quantification method was developed to validate the decolorization/biotransformation of Disperse Orange ERL and dye mixture by lichen Parmelia sp. which release several colored compounds during decolorization process, hence unable to use colorimetric estimation. Percent decolorization of Disperse Orange ERL and dye mixture by lichen Parmelia sp. was observed when estimated using developed HPTLC method. Limit of detection and limit of quantification for both dyes in mixture were obtained as 0.3 and 1 μg/μl, respectively. Area of peak of control Disperse Orange ERL was reduced by 43% after 12 h, 71% after 48 h and upto 82% after 72 h of incubation. Precision and repeatability of data elucidated the % relative standard deviation less than 3 for all the values thus indicating statistically acceptable. Biodegradation of dye and mixture was confirmed with Fourier transform infrared spectroscopy analysis, i.e., altered fingerprinting spectral pattern.
开发了密度测定高效薄层色谱（HPTLC）定量方法，以验证地衣 Parmelia sp. 对分散橙 ERL 和染料混合物的脱色/生物转化。地衣在脱色过程中会释放几种有色化合物，因此无法使用比色法进行估算。使用开发的 HPTLC 方法估算时，观察到地衣 Parmelia sp. 对分散橙 ERL 和染料混合物的脱色百分比。混合物中两种染料的检测限和定量限分别为 0.3 和 1 μg/μl。孵育 12 小时后，对照分散橙 ERL 的峰面积减少了 43%，48 小时后减少了 71%，72 小时后减少了 82%。数据的精密度和重复性表明所有值的相对标准偏差百分比均小于 3，因此在统计学上是可接受的。通过傅里叶变换红外光谱分析证实了染料和混合物的生物降解，即指纹光谱图发生了改变。
