College of Life Science, Xinyang Normal University, Xinyang 464000, China; Tea Plant Biology Key Laboratory of Henan Province, Xinyang 464000, China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang 464000, China.
College of Life Science, Xinyang Normal University, Xinyang 464000, China.
Int J Biol Macromol. 2018 Dec;120(Pt B):2175-2179. doi: 10.1016/j.ijbiomac.2018.09.041. Epub 2018 Sep 8.
Calcium carbonate/xanthan gum (CaCO/XG) microspheres were prepared using biomimetic mineralization method for lysozyme (Ly) adsorption. The morphology of CaCO/XG microspheres was characterized by field emission scanning electron microscope (FE-SEM). The Ly adsorption behavior was verified by Fourier transform infrared (FTIR) and in situ fluorescence microscope images. The effects of pHs on lysozyme adsorption were investigated as well. It was revealed that CaCO/XG microspheres could immobilize lysozyme efficiently via electrostatic interactions with adsorption rate and adsorption quantity of 58.55 ± 0.56% and 18.7 ± 1.2 μg/mg as the pH was 7.0. Comparatively, the values markedly improved to 80.97 ± 0.15% and 24.3 ± 0.1 μg/mg respectively as the pH was 9.0 (p < 0.05). Additionally, UV and fluorescence spectrum showed that Ly maintained its original secondary structure during the adsorption/desorption process. The study therefore demonstrated that CaCO/XG microspheres can be used as a practical and efficient support for Ly adsorption and desorption.
碳酸钙/黄原胶（CaCO/XG）微球采用仿生矿化法制备，用于溶菌酶（Ly）吸附。用场发射扫描电子显微镜（FE-SEM）对 CaCO/XG 微球的形态进行了表征。通过傅里叶变换红外（FTIR）和原位荧光显微镜图像验证了 Ly 的吸附行为。还研究了 pH 对溶菌酶吸附的影响。结果表明，CaCO/XG 微球可以通过静电相互作用有效地固定溶菌酶，吸附率和吸附量分别为 58.55±0.56%和 18.7±1.2μg/mg，pH 值为 7.0。相比之下，当 pH 值为 9.0 时，这些值分别显著提高到 80.97±0.15%和 24.3±0.1μg/mg（p<0.05）。此外，紫外和荧光光谱表明，Ly 在吸附/解吸过程中保持其原始二级结构。因此，该研究表明 CaCO/XG 微球可用作溶菌酶吸附和解吸的实用且高效的载体。
